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An analysis of the proteinases of Trichomonas vaginalis by polyacrylamide gel electrophoresis

Published online by Cambridge University Press:  06 April 2009

G. H. Coombs  and
M. J. North
G. H. Coombs
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ and Department of Biological Science, University of Stirling, Stirling FK9 4LA
M. J. North
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ and Department of Biological Science, University of Stirling, Stirling FK9 4LA
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Summary

The proteinases of Trichomonas vaginalis have been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. Seven bands of activity were detected indicating multiple proteinases. All of the enzymes were stimulated by 1 mM dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. The enzymes differed significantly, however, with respect to pH optima and relative sensitivities to inhibitors.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 1 , February 1983 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1983

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