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An electrophysiological preparation of Ascaris suum pharyngeal muscle reveals a glutamate-gated chloride channel sensitive to the avermectin analogue, milbemycin D

Published online by Cambridge University Press:  26 March 2010

R. J. Martin
R. J. Martin
Affiliation:
Department of Preclinical Veterinary Sciences, R.(D.) S. V. S., Summerhall, University of Edinburgh, Edinburgh EH9 1QT
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Summary

An electrophysiological preparation of Ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. The preparation is shown to contain a glutamate-gated Cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin D. The application of glutamate produces a dose-dependent increase in Cl conductance and the effect of glutamate is potentiated by milbemycin D. Milbemycin D also produced a dose-dependent increase in input conductance.

Keywords

glutamateavermectinmilbemycinchloride channelanthelminticAscaris suum
Type
Research Article
Information
Parasitology , Volume 112 , Issue 2 , February 1996 , pp. 247 - 252
Copyright
Copyright © Cambridge University Press 1996

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References

Arena, J. P. (1994). Expression of Caenorhabditis elegans messenger-RNA in xenopus oocytes: a model system to study the mechanism of action of avermectins. Parasitology Today 10, 35–7.CrossRefGoogle Scholar
Avery, L. (1993). Motor-neuron m3 controls pharyngeal muscle-relaxation timing in Caenorhabditis elegans. Journal of Experimental Biology 175, 283–97.CrossRefGoogle ScholarPubMed
Avery, L., Davis, M. W., Denk, W., Dent, J. & Hess, G. (1994). M3 is an inhibitory glutamatergic pharyngeal motoneuron. Worm Breeder's Gazette 13, 72.Google Scholar
Byerly, L. & Masuda, M. O. (1979). Voltage-clamp analysis of the potassium current that produces a negative-going action potential in Ascaris muscle. Journal of Physiology 288, 263–84.CrossRefGoogle ScholarPubMed
Campbell, W. C. & Benz, G. W. (1984). Ivermectin: a review of efficacy and safety. Journal of Veterinary Pharmacology and Therapeutics 7, 1–16.CrossRefGoogle ScholarPubMed
Cully, D. F., Vassilatis, D. K., Liu, K. K., Paress, P. S., Vanderploeg, L. H. T. & Schaeffer, J. M. (1994). Cloning of an avermectin-sensitive glutamate-gated chloride channel from Caenorhabditis elegans. Nature, London 371, 707–11.CrossRefGoogle Scholar
Del Castillo, J. & Morales, T. (1967). The electrical and mechanical activity of the esophageal cell of Ascaris lumbricoides. Journal of Generative Physiology 50, 603–30.CrossRefGoogle ScholarPubMed
Geary, T. G., Sims, S. M., Thomas, E. M., Vanover, L., Davis, J. P., Winterrowd, C. A. & Klein, R. (1993). Haemonchus contortus ivermectin-induced paralysis of the pharynx. Experimental Parasitology 77, 88–96.CrossRefGoogle ScholarPubMed
Holden-Dye, L. & Walker, R. J. (1990). Avermectin and avermectin derivatives are antagonists at the 4-aminobutyric acid (GABA) receptors on the somatic muscle cells of Ascaris: is this the site of anthelmintic action? Parasitology 101, 265–71.CrossRefGoogle Scholar
Horseman, B. G., Seymour, C., Bermudez, I. & Beadle, D. J. (1988). The effects of L-glutamate on cultured insect neurons. Neuroscience Letters 85, 65–70.CrossRefGoogle Scholar
Laughton, D. L., Wolstenholme, A. J. & Lunt, G. (1995). The beta subunit of the avermectin sensitive glutamate receptor is expressed in the pm4 pharyngeal muscle cells. Worm Breeder's Gazette 14, 48.Google Scholar
Lea, T. J. & Usherwood, P. N. R. (1973). The site of action of ibotenic acid and the identification of two populations of glutamate receptors on insect muscle-fibres. Comparative and General Pharmacology 4, 333–50.CrossRefGoogle ScholarPubMed
Lingle, C. & Marder, E. (1981). A glutamate-activated chloride conductance on a crustacean muscle. Brain Research 212, 481–8.CrossRefGoogle ScholarPubMed
Martin, R. J. & Pennington, A. J. (1989). A patch-clamp study of effects of dihydroavermectin on Ascaris muscle. British Journal of Pharmacology 98, 747–56.CrossRefGoogle ScholarPubMed
Wafford, K. A. & Sattelle, D. B. (1989). l-glutamate receptors on the cell body membrane of an identified insect motor neuron. Journal of Experimental Biology 144, 449–62.CrossRefGoogle Scholar