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Analysis of Opisthorchis viverrini antigens by immunoprecipitation and polyacrylamide gel electrophoresis

Published online by Cambridge University Press:  06 April 2009

S. Wongratanacheewin ,
R. Chawengkirttikul ,
D. Bunnag  and
S. Sirisinha
S. Wongratanacheewin
Affiliation:
Department of Microbiology, Faculty of Science, and Hospital for Tropical Diseases
R. Chawengkirttikul
Affiliation:
Department of Microbiology, Faculty of Science, and Hospital for Tropical Diseases
D. Bunnag
Affiliation:
Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
S. Sirisinha
Affiliation:
Department of Microbiology, Faculty of Science, and Hospital for Tropical Diseases
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Summary

Antigenicity of adult Opisthorchis viverrini, metacercariae and eggs was investigated by radio-immunoprecipitation and polyacrylamide gel electrophoresis using sera from patients with opisthorchiasis and from hamsters experimentally infected with 0. viverrini metacercariae. 125I-labelled aqueous somatic extract and in vitro metabolic products of adult worms, and the extracts of metacercariae and eggs were immunoprecipitated, electrophoresed and autoradiographed and major antigens were characterized. Although components with Mr of > 116, 89, 78 and 20 kD appeared to be specifically associated with the somatic extract of adult fluke, the 89 kD protein was the most prominent component found in the in vitro culture fluid of adult worms and the metacercarial extract. Hamsters, experimentally infected with 0. viverrini, also showed the presence of antibody reactive with the 89 kD component within 15 days of infection. The potential of this antigen in immunodiagnosis is discussed.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 119 - 128
Copyright
Copyright © Cambridge University Press 1988

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