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Catabolism of deoxythymidylate in some trypanosomatids

Published online by Cambridge University Press:  06 April 2009

K. Al Chalabi  and
W. E. Gutteridge
K. Al Chalabi
Affiliation:
Biological Laboratory, University of Kent, Canterbury, Kent CT2 7NJ
W. E. Gutteridge
Affiliation:
Biological Laboratory, University of Kent, Canterbury, Kent CT2 7NJ
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Extract

An initial observation converning the failure of [3H]thymidine at high specific activity to be incorporated into the DNA of Crithidia fasciculata for more than a brief intitial period has been correlated with the presence at high specific activity in the organism of thymidine phosphorylase activity with an equilibrium in the direction of catabolism. This enzyme degrades thymidine to thymine which is not utilized by the organism. The enzyme has also been shown to be present in a number of other trypanosomatids, including the culture forms of Trypanosoma cruzi, where the specific activity was nearly as high as that in C. fasciculata.

Evidence is presented that in C. fasciculata, the culture forms of T. cruzi and possibly other species of trypanosomatid, the thymidine phosphorylase, together with a thymidylate phosphatase, forms a catabolic pathway which degrades thymine nucleotides to thymine, which is then excreated. About 60% of the thymine nucleotides made by organisms appear to be matabolized through the pathway, suggesting that their synthesis is not subject to completely effective regulatory control.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 3 , June 1977 , pp. 299 - 312
Copyright
Copyright © Cambridge University Press 1977

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