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Characterization of the first European isolate of Neospora caninum (Dubey, Carpenter, Speer, Topper and Uggla)

Published online by Cambridge University Press:  06 April 2009

J. S. Barber ,
O. J. M. Holmdahl ,
M. R. Owen ,
F. Guy ,
A. Uggla  and
A. J. Trees
J. S. Barber
Affiliation:
Veterinary Parasitology, Liverpool School of Tropical Medicine and Faculty of Veterinary Science, University of Liverpool, Pembroke Place, Liverpool L3 5QA, UK
O. J. M. Holmdahl
Affiliation:
Department of Parasitology, National Veterinary Institute and Swedish University of Agricultural Sciences, Box 7073, S-750 07, Uppsala, Sweden
M. R. Owen
Affiliation:
Veterinary Parasitology, Liverpool School of Tropical Medicine and Faculty of Veterinary Science, University of Liverpool, Pembroke Place, Liverpool L3 5QA, UK
F. Guy
Affiliation:
Veterinary Parasitology, Liverpool School of Tropical Medicine and Faculty of Veterinary Science, University of Liverpool, Pembroke Place, Liverpool L3 5QA, UK
A. Uggla
Affiliation:
Department of Parasitology, National Veterinary Institute and Swedish University of Agricultural Sciences, Box 7073, S-750 07, Uppsala, Sweden
A. J. Trees
Affiliation:
Veterinary Parasitology, Liverpool School of Tropical Medicine and Faculty of Veterinary Science, University of Liverpool, Pembroke Place, Liverpool L3 5QA, UK
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Summary

Neospora caninum is an apicomplexan, protozoan parasite, which causes severe disease in dogs and cattle. It has previously been isolated only in the United States. A 5-week-old Boxer pup with a progressive hindlimb paresis was diagnosed as suffering from neosporosis on the basis of clinical signs and the presence of anti-Neospora antibodies in it, 2 litter-mates and its darn. Despite treatment with sulphonamides, the pup was euthanased 3 days later. The diagnosis of neosporosis was confirmed by immunohistochemical examination of muscle and CNS tissue sections from the pup. Parasites were isolated into Vero cell culture from the cerebrum, and confirmed as Neospora caninum by immunofluorescence with specific antibody, tachyzoite ultrastructure and 16S-like ribosomal RNA sequences. This isolate (designated NC-Liverpool) has been continuously passaged every 7–10 days. Its growth characteristics, ultrastructure and antigenic profile, as revealed by immunoblotting, have revealed no major differences from the American NC-1 isolate. Furthermore, no difference was seen when comparing the sequences of 16S-like ribosomal RNA and the ITS1 region of the two isolates.

Keywords

Neospora caninumin vitro isolationdogrRNA
Type
Research Article
Information
Parasitology , Volume 111 , Issue 5 , December 1995 , pp. 563 - 568
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Barta, J. R. & Dubey, J. P. (1992). Characterisation of anti-Neospora caninum hyperimmune rabbit serum by Western blot analysis and immunoelectron microscopy. Parasitology Research 78, 689–94.CrossRefGoogle ScholarPubMed
Bjerkas, I., Jenkins, M. C. & Dubey, J. P. (1994). Identification and characterisation of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clinical and Diagnostic Laboratory Immunology 1, 214–21.CrossRefGoogle ScholarPubMed
Bjerkas, I., Mohn, S. F. & Presthus, J. (1984). Unidentified cyst-forming sporozoon causing encephalitis and myositis in dogs. Zeitschrift für Parasitenkunde 70, 271–4.CrossRefGoogle Scholar
Conrad, P. A., Barr, B. C., Sverlow, K. W., Anderson, M., Daft, B., Kinde, H., Dubey, J. P., Munson, L. & Ardans, A. (1993). In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses. Parasitology 106, 239–49.CrossRefGoogle ScholarPubMed
Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387–95.CrossRefGoogle ScholarPubMed
Dubey, J. P.Carpenter, J. L., Speer, C. A., Topper, M. J. & Uggla, A. (1988 a). Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association 192, 1269–85.Google ScholarPubMed
Dubey, J. P., Hattel, A. L., Lindsay, D. S. & Topper, M. J. (1988 b). Neonatal Neospora caninum infection in dogs: isolation of the causative agent and experimental transmission. Journal of the American Veterinary Medical Association 193, 1259–63.Google ScholarPubMed
Dubey, J. P. & Lindsay, D. S. (1993). Neosporosis. Parasitology Today 9, 452–8.CrossRefGoogle ScholarPubMed
Ellis, J., Luton, K. P., Baverstock, R., Brindley, P. J., Nimmo, K. A. & Johnson, A. M. (1994). The phylogeny of Neospora caninum. Molecular and Biochemical Parasitology 64, 303–11.CrossRefGoogle ScholarPubMed
Gajadhar, A. A., Marquardt, W. C., Hall, R., Gunderson, J., Ariztia-Carmona, E. V. & Sogin, M. L. (1991). Ribosomal RNA sequences of Sarcocystis muris, Theileria annulata and Crypthecodinium cohnii reveal evolutionary relationships among apicomplexan dinoflagellates and ciliates. Molecular and Biochemical Parasitology 45, 147–54.CrossRefGoogle ScholarPubMed
Hay, W. H., Shell, L. G., Lindsay, D. S. & Dubey, J. P. (1990). Diagnosis and treatment of Neospora caninum infection in a dog. Journal of the American Veterinary Medical Association 197, 87–9.CrossRefGoogle Scholar
Holmdahl, O. J. M., Mattsson, J. G., Johansson, K.-E. & Uggla, A. (1994). The phylogeny of Neospora caninum and Toxoplasma gondii based on ribosomal RNA sequences. FEMS Microbiology Letters 119, 187–92.CrossRefGoogle ScholarPubMed
Lindsay, D. S. & Dubey, J. P. (1989). Immunohisto-chemical diagnosis of Neospora caninum in tissue sections. American Journal of Veterinary Research 50, 1981–3.Google Scholar
Lindsay, D. S. & Dubey, J. P. (1990). Effects of sulfadiazine and amprolium on Neospora caninum (Protozoa: Apicomplexa) infections in mice. Journal of Parasitology 76, 177–9.CrossRefGoogle ScholarPubMed
Lindsay, D. S., Dubey, J. P. & Blagburn, B. L. (1991). Characterisation of a Neospora caninum (Protozoa: Apicomplexa) isolate (NC-3) in mice. Journal of the Alabama Academy of Science 62, 1–8.Google Scholar
Lindsay, D. S., Dubey, J. P., Upton, S. J. & Ridley, R. K. (1990). Serological prevalence of Neospora caninum and Toxoplasma gondii in dogs from Kansas. Journal of the Helminthological Society of Washington 57, 86–8.Google Scholar
Lindsay, D. S., Rippey, N. S., Cole, R. A., Parsons, L. C., Dubey, J. P., Tidwell, R. R. & Blagburn, B. L. (1994). Examination of the activities of 43 chemotherapeutic agents against Neospora caninum tachyzoites in cultured cells. American Journal of Veterinary Research 55, 976–81.CrossRefGoogle ScholarPubMed
Lindsay, D. S., Speer, C. A., Toivio-Kinnucan, M. A., Dubey, J. P. & Blagburn, B. L. (1993). Use of infected cultured cells to compare ultrastructural features of Neospora caninum from dogs and Toxoplasma gondii. American Journal of Veterinary Research 54, 103–6.CrossRefGoogle ScholarPubMed
Speer, C. A. & Dubey, J. P. (1989). Ultrastructure of tachyzoites, bradyzoites and tissue cysts of Neospora caninum. Journal of Protozoological Research 36, 458–63.CrossRefGoogle ScholarPubMed
Trees, A. J., Guy, F., Low, J. C., Roberts, L., Buxton, D. & Dubey, J. P. (1994). Serological evidence implicating Neospora species as a cause of abortion in British cattle. Veterinary Record 134, 405–7.CrossRefGoogle ScholarPubMed
Trees, A. J., Guy, F., Tennant, B. J., Balfour, A. H. & Dubey, J. P. (1993). Prevalence of antibodies to Neospora caninum in an English urban dog population. Veterinary Record 132, 125–6.CrossRefGoogle Scholar