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Chronic indeterminate phase of Chagas’ disease: mitochondrial involvement in infection with two strains

Published online by Cambridge University Press:  09 November 2012

ALEJANDRA LIDIA BÁEZ ,
MARÍA SILVINA LO PRESTI ,
RICARDO FRETES ,
CINTIA DÍAZ ,
PATRICIA PONS ,
PAOLA CAROLINA BAZÁN ,
MARIANA STRAUSS ,
HÉCTOR WALTER RIVAROLA  and
PATRICIA PAGLINI-OLIVA
ALEJANDRA LIDIA BÁEZ*
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
MARÍA SILVINA LO PRESTI
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
RICARDO FRETES
Affiliation:
Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. 5016 Córdoba, Argentina
CINTIA DÍAZ
Affiliation:
Cátedra de Biología Celular, Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. 5016 Córdoba, Argentina
PATRICIA PONS
Affiliation:
Centro de Microscopia, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. 5016 Córdoba, Argentina
PAOLA CAROLINA BAZÁN
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
MARIANA STRAUSS
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
HÉCTOR WALTER RIVAROLA
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
PATRICIA PAGLINI-OLIVA
Affiliation:
Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Santa Rosa 1085. PC 5000. Córdoba, Argentina
*
*Corresponding author: Cátedra de Física Biomédica,Facultad de Ciencias Médicas. Universidad Nacional de Córdoba, Santa Rosa 1085. C.P. 5000, Argentina. Tel: + 51 9 351 4332020. E-mail address: alejandralidiab@hotmail.com.
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Summary

Chagasic cardiopathy has become one of the most frequent causes of heart failure and sudden death, as well as one of the most common causes of cardio-embolic stroke in Latin America. The myocyte response to oxidative stress involves the progression of cellular changes, primarily targeting the mitochondria and modifying therefore the energy supply. In this paper we analysed the effect of the infection of mice with 2 different strains of Trypanosoma cruzi (Tulahuen and SGO Z12) in the chronic indeterminate stage (75 days post-infection), upon the structure and function of cardiac mitochondria. The structural results showed that 83% of the mitochondria from the Tulahuen-infected mice presented an increase in their matrix and 91% of the mitochondria from the SGO Z12-infected group showed a reduction in their diameter (P < 0·05). When the Krebs cycle and mitochondrial respiratory chain functionality was analysed through the measurement of the citrate synthase and complexes I to IV activity, it showed that their activity was altered in all cases in a similar manner in both infected groups. In this paper we have demonstrated that the chronic indeterminate phase is not ‘silent’ and that cardiac mitochondria are clearly involved in the genesis and progression to the chronic chagasic cardiopathy when different factors alter the host-parasite equilibrium.

Keywords

Chronic indeterminate Chagas' diseaseTrypanosoma cruzi strainsmitochondriarespiratory chainmyocardiopathy
Type
Research Article
Information
Parasitology , Volume 140 , Issue 3 , March 2013 , pp. 414 - 421
Copyright
Copyright © Cambridge University Press 2012

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