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The development of Trypanosoma cruzi in macrophages in vitro. Interaction with lysosomes and host cell fate

Published online by Cambridge University Press:  06 April 2009

Regina Milder  and
Judith Kloetzel
Regina Milder
Affiliation:
Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, 01000 São Paulo, Brasil
Judith Kloetzel
Affiliation:
Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, 01000 São Paulo, Brasil
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Summary

The interaction between mouse peritoneal macrophages and ‘Y’ strain Trypanosoma cruzi bloodstream forms was studied at optical and electron microscopical levels. The method of marking lysosomes with Thorotrast, either before or after infection of cell monolayers with parasites, revealed that secondary lysosomes fused with phagosomes shortly after trypanosome interiorization. In spite of this, 24 h later most parasites were no longer in a vacuole but lay free within the host cell cytoplasm, multiplying actively. At this time, and up to shortly before 96 h when parasites escaped to the external milieu, most parasitized cells were not lethally injured, as revealed by the Trypan blue dye-exclusion test. Only when parasites were released into the external medium was this situation reversed and infected macrophages took up the dye.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 139 - 145
Copyright
Copyright © Cambridge University Press 1980

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