Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-10T17:05:51.514Z Has data issue: false hasContentIssue false
  • English
  • Français

Differential diagnosis of Taenia saginata and Taenia solium with DNA probes

Published online by Cambridge University Press:  06 April 2009

L. J. S. Harrison ,
J. Delgado  and
R. M. E. Parkhouse
L. J. S. Harrison
Affiliation:
Centre for Tropical Vetrinary Medicine, Department of Tropical Animal HealthUniversity of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG
J. Delgado
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
R. M. E. Parkhouse
Affiliation:
Division of Immunology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Summary

A size selected genomic DNA library was constructed using DNA extracted from Taenia saginata. The DNA was digested using the restriction enzyme EcoR1 under star conditions and the 2–4 kbase fraction, selected following sucrose density-gradient separation, was cloned in the bacteriophage λgt 10. A panel of cestode DNAs including Taenia saginata, Taenia solium, Taenia taeniaeformis, Taenia crassiceps, Echinococcus granulosus and DNAs of bovine, porcine and human origin were used in conjunction with hybridization analysis to identify two recombinant bacteriophages. The first probe, designated HDP1, reacted specifically with T. saginata DNA. The second, designated HDP2, reacted with DNA from both T. saginata and T. solium but not the other DNA samples and thus provided a general reagent for positive identification of fragments of Taenia spp. proglottides of human faecal origin. If used in conjunction the two DNA probes allow positive identification of T. saginata. In the clinical situation it is important to be able to distinguish T. saginata and T. solium infections and DNA probes such as these may be useful in such differentiation.

Keywords

Taenia saginataTaenia solium differentiationDNA probeshybridization
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 459 - 461
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barker, D. C. (1989). Molecular approaches to DNA diagnosis. Parasitology 99, S125–S146.CrossRefGoogle ScholarPubMed
Bursey, C. C.McKenzie, J. A. & Burt, M. B. D. (1980). Polyacrylamide gel electrophoresis in the identification of Taenia (Cestoda) by total protein. International Journal for Parasitology 10, 167–74.CrossRefGoogle Scholar
Fan, P. C. (1988). Taiwan Taenia and Taeniasis. Parasitology Today 4, 86–8.CrossRefGoogle ScholarPubMed
Flisser, A., Willms, K., Laclette, I. P., Larralde, C., Ridaura, C. & Beltran, F. (1982). Cysticercosis: Present State of Knowledge and Perspectives. London: Academic Press.Google Scholar
Harnett, W.Chambers, A. E.Renz, A. & Parkhouse, R. M. E. (1989). An oligonucleotide probe specific for Onchocerca volvulus. Molecular and Biochemical Parasitology 35, 119–26.CrossRefGoogle ScholarPubMed
Le Riche, P. D. & Sewell, M. M. H. (1977). Differentiation of Taenia saginata and Taenia solium by enzyme electrophoresis.. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 237–8.CrossRefGoogle ScholarPubMed
Le Riche, P. D. & Sewell, M. M. H. (1978). Differentiation of taeniid cestodes by enzyme electrophoresis.. International Journal for Parasitology 8, 476–83.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning. New York: Cold Spring Harbour Publications.Google Scholar
Proctor, E. M. (1972). Identification of tapeworms. South African Medical Journal 46, 234–8.Google ScholarPubMed
Rigby, P. W. J.Dieckmann, M.Rhodes, C. & Berg, P. (1977). Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.. Journal of Molecular Biology 113, 237–51.CrossRefGoogle ScholarPubMed
Rishi, A. K. & McManus, D. P. (1987). DNA probes which unambiguously distinguish Taenia solium from Taenia saginata. The Lancet, 1275–6.CrossRefGoogle Scholar
Rishi, A. K. & McManus, D. P. (1988). Molecular cloning of T. solium genomic DNA and characterization of taeniid cestodes by DNA analysis.. Parasitology 97, 161–76.CrossRefGoogle ScholarPubMed
Soulsby, E. J. L. (1982). Helminths, Arthropods and Protozoa of Domesticated Animals, 7th Edn.London: Bailliere and Tyndall.Google Scholar