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Distribution of schistosome genetic diversity within naturally infected Rattus rattus detected by RAPD markers

Published online by Cambridge University Press:  06 April 2009

V. Barral ,
S. Morand ,
J. P. Pointier  and
A. Théron
V. Barral*
Affiliation:
Laboratoire de Biologie Animale, UMR 5555 du CNRS, Centre de Biologie et d'Ecologie tropicale et méditerranéenne, Université 52, Av. de Villeneuve, 66860 Perpignan Cedex, France
S. Morand
Affiliation:
Laboratoire de Biologie Animale, UMR 5555 du CNRS, Centre de Biologie et d'Ecologie tropicale et méditerranéenne, Université 52, Av. de Villeneuve, 66860 Perpignan Cedex, France
J. P. Pointier
Affiliation:
Laboratoire de Biologie Marine et Malacologie, Ecole Pratique des Hantes Etudes, Centre de Biologie et d'Ecologie tropicale et méditerranéenne, Université 52, Av. de Villeneuve, 66860 Perpignan Cedex, France
A. Théron
Affiliation:
Laboratoire de Biologie Animale, UMR 5555 du CNRS, Centre de Biologie et d'Ecologie tropicale et méditerranéenne, Université 52, Av. de Villeneuve, 66860 Perpignan Cedex, France
*
* Corresponding author. Laboratoire de Biologie Animale, UMR 5555 du CNRS, Centre de Biologie et d'Ecologie tropicale et méditerranéenne, Université, 52 Av. de Villeneuve, 66860 Perpignan Cedex, France. Tel: 33 68 66 21 83. Fax: 33 68 66 22 81. E-mail: parasite@univperp.fr.
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Summary

Random amplified polymorphic DNA markers (RAPD) were used to visualize the genetic diversity within and between infrapopulations of Schistosoma mansoni recovered from the natural vertebrate host, Rattus rattus, trapped at an insular Guadeloupean focus. Phenotypes were characterized by the sex of the parasites and by 8 polymorphic markers generated by 3 primers. Among the 212 parasite individuals recovered from 10 infected rats, 78 genotypes were characterized. All the hosts naturally infected harboured multiple parasite genotypes with a maximum diversity of 28 genotypes/host. Phenotypic and genotypic diversity calculated by Shannon-Wiener's indices and Lynch and Milligan's estimators respectively is, on average, greater within than between hosts. Considering the very low snail infection rates observed in this focus and the rapid turnover of the vertebrate hosts, our results suggest that the high mobility of the vertebrate host and/or plurimiracidial snail infections could be factors responsible for parasite genetic diversity within hosts.

Keywords

genetic diversityvertebrate hostSchistosoma mansoniRattus rattusRAPDparasite population
Type
Research Article
Information
Parasitology , Volume 113 , Issue 6 , December 1996 , pp. 511 - 517
Copyright
Copyright © Cambridge University Press 1996

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