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Effect of environmental factors on survival and population growth of ciliated parasite, Mesanophrys sp. (Ciliophora: Scuticociliatia) infecting Portunus trituberculatus

Published online by Cambridge University Press:  04 November 2020

Summia Perveen ,
Yuhua Lei ,
Fei Yin Open the ORCID record for Fei Yin [Opens in a new window]  and
Chunlin Wang
Summia Perveen
Affiliation:
Key Laboratory of Applied Marine Biotechnology, Ministry of Education; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture; School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo315211, P. R. China
Yuhua Lei
Affiliation:
Key Laboratory of Applied Marine Biotechnology, Ministry of Education; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture; School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo315211, P. R. China
Fei Yin*
Affiliation:
Key Laboratory of Applied Marine Biotechnology, Ministry of Education; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture; School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo315211, P. R. China
Chunlin Wang
Affiliation:
Key Laboratory of Applied Marine Biotechnology, Ministry of Education; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture; School of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo315211, P. R. China
*
Author for correspondence: Fei Yin, E-mail: Yinfei@nbu.edu.cn; Chunlin Wang, E-mail: Wangchunlin@nbu.edu.cn
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Abstract

Mesanophrys sp. is a newly identified parasitic ciliate infecting farmed swimming crab. To demonstrate the correlation between parasite development and environmental conditions, this study aimed to investigate the effect of temperature, salinity, pH and frequency of passage of parasite on survival, growth and body size of Mesanophrys sp. in vitro. The results revealed that survival, population density and growth rate of the parasite were highest at 12°C and decreased with increasing temperature from 16 to 26°C. In addition, the survival, population density and growth rate of Mesanophrys sp. were high at 20‰. When salinity was adjusted to levels lower (0–10‰) and higher (40–60‰) than 20‰, the parasite's survival and growth rate gradually declined. The optimal pH for parasite survival was 8.0, whereas its survival was inhibited at <4.5 or >9.5. Our result also showed that parasite body proportions (length:width) were significantly smaller at the highest temperature compared to the lower temperature, whereas different salinities had no significant effect. Furthermore, we introduced dynamic parasite culture systems in vitro where Mesanophrys sp. was cultured in medium-containing culture plates through continually reducing and halving the old medium into fresh. Application of this optimized dilution timing technique with fresh medium and sub-cultured enabled a continuous culture of parasites. Under this optimized condition, the highest population density and exponential growth rate of the parasite were achieved than that of a control group. This study will help to understand the ciliated parasite infection dynamics and provides new possibilities for in vitro parasite-associated studies.

Keywords

Mesanophrys sp.pHpopulation growthsalinityscuticociliatessurvivaltemperature
Type
Research Article
Information
Parasitology , Volume 148 , Issue 4 , April 2021 , pp. 477 - 485
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press.

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Footnotes

*

The authors contributed equally to this work.

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