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Effect of GSK-3 activity, enzymatic inhibition and gene silencing by RNAi on tick oviposition and egg hatching

Published online by Cambridge University Press:  26 May 2010

ARIANNE FABRES ,
CAROLINE PINTO DE ANDRADE ,
MELINA GUIZZO ,
MARCOS HENRIQUE F. SORGINE ,
GABRIELA DE O. PAIVA-SILVA ,
AOI MASUDA ,
ITABAJARA DA SILVA VAZ Jr  and
CARLOS LOGULLO
ARIANNE FABRES
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos – CBB – UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ, Brazil, CEP 28015-620
CAROLINE PINTO DE ANDRADE
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre, RS, 91501-970, Brazil
MELINA GUIZZO
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre, RS, 91501-970, Brazil
MARCOS HENRIQUE F. SORGINE
Affiliation:
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
GABRIELA DE O. PAIVA-SILVA
Affiliation:
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
AOI MASUDA
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre, RS, 91501-970, Brazil Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
ITABAJARA DA SILVA VAZ Jr
Affiliation:
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, Prédio 43421, Porto Alegre, RS, 91501-970, Brazil Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
CARLOS LOGULLO*
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos – CBB – UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ, Brazil, CEP 28015-620
*
*Corresponding author: Laboratório de Química e Função de Proteínas e Peptídeos – CBB – UENF, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacazes, RJ, Brazil, CEP 28015-620. Tel: + 55 22 27261467. Fax: +55 22 27261520. E-mail: logullo@uenf.br
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Summary

Glycogen synthase kinase-3 (GSK-3) is classically described as a key enzyme involved in glycogen metabolism in mammals. It has been shown to be highly conserved among several organisms, mainly in the catalytic domain region. This enzyme has already been described in Rhipicephalus (Boophilus) microplus and the ovaries of females appeared to be the major site of GSK-3 transcription. The treatment with GSK-3 specific inhibitor (alsterpaullone, bromo-indirubin-oxime 6 and indirubin-3-oxime) caused a reduction in oviposition and egg hatching in completely engorged female ticks. The effect was more pronounced in partially engorged females when alsterpaullone was administrated by artificial capillary feeding. Moreover, GSK-3 gene silencing by RNAi in partially engorged females reduced significantly both oviposition and hatching. The study of tick embryogenesis and proteins that participate in this process has been suggested as an important means for the development of novel strategies for parasite control. GSK-3 is an essential protein involved in embryonic processes and for this reason it has already been suggested as a possible antigen candidate for tick control.

Keywords

GSK-3tickinhibitorsRNAiembryogenesishatching
Type
Research Article
Information
Parasitology , Volume 137 , Issue 10 , September 2010 , pp. 1537 - 1546
Copyright
Copyright © Cambridge University Press 2010

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