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Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi

Published online by Cambridge University Press:  22 February 2007

A. CICCARELLI ,
L. ARAUJO ,
A. BATLLE  and
E. LOMBARDO
A. CICCARELLI
Affiliation:
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Argentina
L. ARAUJO
Affiliation:
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Argentina Departamento de Química Biológica, FCEN-UBA, Argentina
A. BATLLE*
Affiliation:
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Argentina
E. LOMBARDO
Affiliation:
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-UBA, Argentina Departamento de Química Biológica, FCEN-UBA, Argentina
*
*Corresponding author: Viamonte 1881, 10 “A”, CP-1056 Buenos Aires, Argentina. Tel: +5411 4812 3357. Fax: +5411 4811 7447. E-mail: batlle@mail.retina.ar
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Summary

A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi was evaluated. We have observed that, in epimastigotes grown under different haemin concentrations in the culture medium (0–30 mg/l), 5 mg/l was the haemin concentration yielding optimum growth. Above 15 mg/l there was a clear decrease in growth rate, producing the epimastigote to amastigote transformation. Such morphological change was observed together with a marked injury of the enzymatic machinery of the parasite, leading to diminished protein synthesis as well as lower activity of the antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and trypanothione reductase), reduced total thiol content and a marked increase in the HaemOx-1 activity and expression. The current work demonstrates that there is a correlation between higher haemin concentrations in the culture medium and oxidative damage in the cells. Under these conditions induction of HaemOx-1 would indicate the important role of this enzyme as an antioxidant defence response in Trypanosoma cruzi.

Keywords

Trypanosoma cruzihaeminantioxidant enzymescell transformationhaeme oxygenase
Type
Research Article
Information
Parasitology , Volume 134 , Issue 7 , July 2007 , pp. 959 - 965
Copyright
Copyright © Cambridge University Press 2007

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