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Enzyme variation in Trypanosoma brucei spp. I. Evidence for the sub-speciation of Trypanosoma brucei gambiense

Published online by Cambridge University Press:  06 April 2009

A. Tait ,
Eldirdiri A. Babiker  and
D. Le Ray
A. Tait
Affiliation:
Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh, Scotland and Institute of Tropical Medicine Prince Leopold, Laboratory of Protozoology, B-2000 Antwerpen, Belgium
Eldirdiri A. Babiker
Affiliation:
Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh, Scotland and Institute of Tropical Medicine Prince Leopold, Laboratory of Protozoology, B-2000 Antwerpen, Belgium
D. Le Ray
Affiliation:
Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh, Scotland and Institute of Tropical Medicine Prince Leopold, Laboratory of Protozoology, B-2000 Antwerpen, Belgium
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Summary

Three groups of stocks of Trypanosoma brucei ssp, defined by the criteria of host, human serum resistance and place of isolation as T. b. gambiense, T. b. brucei (Nigeria) and T. b. brucei/rhodesiense (non-gambiense, Uganda) were screened for electrophoretic variation at 20 enzyme loci. One enzyme (Peptidase C) was found to differentiate all T. b. gambiense stocks from the other T. brucei stocks and, taken together with specific variants of 5 other enzymes, could be used to unambiguously define T. b. gambiense stocks. Using a population genetics approach, the frequencies of the different variants in the three groups of stocks were estimated and from them the average similarity and difference between groups were measured using the statistics of genetic identity (I) and genetic distance (D). These results show firstly, that T. b. gambiense is more different from the other two groups than they are from each other and, secondly, that the values of I and D obtained are consistent with T. b. gambiense constituting a sub- or sibling species of T. brucei. Three domestic animal isolates from Zaire and Cameroun were also screened for enzyme variation and two of these identified as T. b. gambiense, thereby establishing the existence of an animal reservoir host. In parallel, these stocks were tested for human serum resistance resulting in the same identification. Studies of the antigen repertoires and antigen gene structure were carried out by other workers on all the T. b. gambiense stocks reported here and the same conclusions reached as to the identification and ability to discriminate this subspecies from other groups of T. brucei stocks. The results presented here are discussed in relation to other published data on enzyme variation in T. brucei.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 2 , October 1984 , pp. 311 - 326
Copyright
Copyright © Cambridge University Press 1984

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References

Apted, F. I. C. (1970). The epidemiology of Rhodesian sleeping sickness. In The African Trypanosomiases (ed. Mulligan, H. W.), pp, 645–660. London: Allen & Unwin.Google Scholar
Ayala, F. J., Tracy, M. L., Hedgecock, D. & Richmond, R. C. (1974). Genetic differentiation during the speciation process in Drosophila. Evolution 28, 576–92.CrossRefGoogle ScholarPubMed
Borst, P., Van der Ploeg, M., Van Hoek, J. F. M., Tas, J. & James, J. (1982). On the DNA content and ploidy of trypanosomes. Molecular and Biochemical Parasitology 6, 13–23.CrossRefGoogle ScholarPubMed
Eldirdiri, A. B. & Le Ray, D. (1981). Adaptation of low virulence stocks of T. b. gambiense to rat and mouse. Annales de la Société belge de médecine tropicale 61, 51–9.Google Scholar
Ferguson, A. (1980). Biochemical Systematics and Evolution. Glasgow and London: Blackie.Google Scholar
Gibson, W., Marshall, T. F. De C. & Godfrey, D. G. (1980). Numerical analysis of enzyme polymorphism: a new approach to the epidemiology and taxonomy of trypanosomes of the subgenus Trypanozoon. Advances in Parasitology 18, 175–246.CrossRefGoogle Scholar
Gibson, W., Mehlitz, D., Lanham, S. M. & Godfrey, D. G. (1978). The identification of T. brucei gambiense in Liberian pigs and dogs by isoenzymes and resistance to human plasma. Tropenmedizin und Parasitologie 29, 335–45.Google ScholarPubMed
Godfrey, D. G. & Kilgour, V. (1976). Enzyme electrophoresis in characterising the causative organism of Gambian trypanosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 219–24.CrossRefGoogle ScholarPubMed
Harris, H. & Hopkinson, D. A. (1976). Handbook of Enzyme Electrophoresis in Human Genetics. Amsterdam: North Holland Publishing Company.Google Scholar
Hawking, F. (1976). The resistance to human plasma of Trypanosoma brucei, T. rhodesiense and T. gambiense. II. Survey of strains from East Africa and Nigeria. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 513–20.CrossRefGoogle Scholar
Heisch, R. B., McMahon, J. P. & Manson-Baht, P. E. C. (1958). The isolation of T. rhodesiense from a bushbuck. British Medical Journal 2, 1203–4.CrossRefGoogle Scholar
Hoare, C. A. (1972). The Trypanosomes of Mammals. Oxford: Blackwell Scientific Publications.Google Scholar
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other animals using DEAE-cellulose. Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
Mayr, E. (1953). Concepts of classification and nomenclature in higher organisms and micro-organisms. Annals of the New York Academy of Sciences 56, 391–7.CrossRefGoogle Scholar
Mehlitz, D., Zillman, U., Scott, G. M. & Godfrey, D. G. (1982). Epidemiological studies on the animal reservoir of Gambiense sleeping sickness. III. Characterization of Trypanosoon stocks by isoenzymes and sensitivity to human serum. Tropenmedizin und Parasitologie 33, 113–18.Google ScholarPubMed
Miles, M. A., Lanham, S. M., De Souza, A. A. & Pova, M. (1980). Further enzymic characters of T. cruzi and their evaluation for strain identification. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 221–37.CrossRefGoogle Scholar
Nei, M. (1972). Genetic distance between populations. American Naturalist 106, 283–92.CrossRefGoogle Scholar
Pays, E., Dekerck, P., Van Assel, S., Sternert, M., Le Ray, D. & Van Meirvenne, N. (1983). Comparative analysis of a T. b. gambiense antigen gene family and its potential use in epidemiology of sleeping sickness. Molecular and Biochemical Parasitology 7, 63–74.CrossRefGoogle Scholar
Rickman, L. R. & Robson, J. (1970). The testing of proven Trypanosoma brucei and T. rhodesiense strains by the blood infectivity test. Bulletin of the World Health Organization 42, 911–16.Google Scholar
Tait, A. (1970). Enzyme variation between syngens in Paramedium aurelia. Biochemical Genetics 4, 461–70.CrossRefGoogle Scholar
Tait, A. (1980). Diploidy and mating in trypanosomes. Nature, London 287, 536–8.CrossRefGoogle ScholarPubMed
Tait, A. (1984). The biochemical characterisation of T. brucei ssp. by enzyme electrophoresis. World Health Organization Symposium on Application of Biochemical and Molecular Biology Techniques to Problems of Parasite and Vector Identification.Geneva: (in the Press).Google Scholar
Tibayrenc, M., Cariou, M. L. & Solignac, M. (1981). Interpretation genetiques des zymogrammes de Flagelle's des genres Trypanosoma et Leishmania. Comptes rendus, Académie des sciences, Paris 292, 623–5.Google Scholar
Tibayrenc, M. & Miles, M. A. (1983). A genetic comparison between Brazilian and Bolivian zymodemes of T. cruzi. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 76–83.CrossRefGoogle Scholar
Van Meirvenne, N., Janssens, P. G. & Magnus, E. (1973). Further studies on the lytic and neutralising action of human serum on T. brucei. Annales de la Société belge de médecine tropicale 53, 49–56.Google ScholarPubMed
Van Meirivenne, N., Janssens, P. G. & Magnus, E. (1975). Antigenic variation in syringe passaged populations of Trypanosoma brucei. 1. Rationalisation of the experimental approach. Annales de la Société belge de médecine tropicale 55, 1–23.Google Scholar
Zimmermann, E. G., Kilpatrick, C. W. & Hart, B. J. (1978). The genetics of speciation in the rodent genus Peromyscus. Evolution 32, 565–79.CrossRefGoogle Scholar