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Exploring metabolomic approaches to analyse phospholipid biosynthetic pathways in Plasmodium

Published online by Cambridge University Press:  29 January 2010

S. BESTEIRO ,
S. VO DUY ,
C. PERIGAUD ,
I. LEFEBVRE-TOURNIER  and
H. J. VIAL
S. BESTEIRO
Affiliation:
Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 107, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France
S. VO DUY
Affiliation:
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 1705, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France
C. PERIGAUD
Affiliation:
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 1705, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France
I. LEFEBVRE-TOURNIER
Affiliation:
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 1705, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France
H. J. VIAL*
Affiliation:
Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 107, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France
*
Corresponding author: Henri Vial, Dynamique des Interactions Membranaires Normales et Pathologiques, UMR 5235, Centre National de la Recherche Scientifique – UM1-UM2, Université Montpellier 2, cc 107, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France. Tel: +33 (0) 4 6714 3745, Fax: +33 (0) 4 6714 4286, E-mail: vial@univ-montp2.fr
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Summary

Plasmodium falciparum, the agent responsible for malaria, is an obligate intracellular protozoan parasite. For proliferation, differentiation and survival, it relies on its own protein-encoding genes, as well as its host cells for nutrient sources. Nutrients and subsequent metabolites are required by the parasites to support their high rate of growth and replication, particularly in the intra-erythrocytic stages of the parasite that are responsible for the clinical symptoms of the disease. Advances in mass spectrometry have improved the analysis of endogenous metabolites and enabled a global approach to identify the parasite's metabolites by the so-called metabolomic analyses. This level of analysis complements the genomic, transcriptomic and proteomic data already available and should allow the identification of novel metabolites, original pathways and networks of regulatory interactions within the parasite, and between the parasite and its hosts. The field of metabolomics is just in its infancy in P. falciparum, hence in this review, we concentrate on the available methodologies and their potential applications for deciphering important biochemical processes of the parasite, such as the astonishingly diverse phospholipid biosynthesis pathways. Elucidating the regulation of the biosynthesis of these crucial metabolites could help design of future anti-malarial drugs.

Keywords

PlasmodiummalariametabolomicsphospholipidsLC-MSmass spectrometry
Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 9: Insights into the metabolomes of parasites , August 2010 , pp. 1343 - 1356
Copyright
Copyright © Cambridge University Press 2010

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