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Genetic complexity and gametocyte production of Plasmodium falciparum in Fulani and Mossi communities in Burkina Faso

Published online by Cambridge University Press:  18 January 2006

G. M. PAGANOTTI ,
C. PALLADINO ,
D. MODIANO ,
B. S. SIRIMA ,
L. RÅBERG ,
A. DIARRA ,
A. KONATÉ ,
M. COLUZZI ,
D. WALLIKER  and
H. A. BABIKER
G. M. PAGANOTTI
Affiliation:
Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK Istituto Pasteur, Fondazione Cenci-Bolognetti, Università di Roma ‘La Sapienza’, P.le Aldo Moro 5, 00185 Rome, Italy
C. PALLADINO
Affiliation:
Dipartimento di Scienze di Sanità Pubblica, Sezione di Parassitologia, Università di Roma ‘La Sapienza’, P.le Aldo Moro 5, 00185 Rome, Italy
D. MODIANO
Affiliation:
Dipartimento di Scienze di Sanità Pubblica, Sezione di Parassitologia, Università di Roma ‘La Sapienza’, P.le Aldo Moro 5, 00185 Rome, Italy
B. S. SIRIMA
Affiliation:
Centre National de Recherche et Formation sur le Paludisme, BP 2208 Ouagadougou, Burkina Faso
L. RÅBERG
Affiliation:
Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
A. DIARRA
Affiliation:
Centre National de Recherche et Formation sur le Paludisme, BP 2208 Ouagadougou, Burkina Faso
A. KONATÉ
Affiliation:
Centre National de Recherche et Formation sur le Paludisme, BP 2208 Ouagadougou, Burkina Faso
M. COLUZZI
Affiliation:
Dipartimento di Scienze di Sanità Pubblica, Sezione di Parassitologia, Università di Roma ‘La Sapienza’, P.le Aldo Moro 5, 00185 Rome, Italy
D. WALLIKER
Affiliation:
Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
H. A. BABIKER
Affiliation:
Institute of Infection and Immunology Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK Department of Biochemistry, Faculty of Medicine, Sultan Qaboos University, Oman
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Abstract

We have examined Plasmodium falciparum gametocyte prevalence, density and their genetic complexity among children of 2 sympatric ethnic groups (Mossi and Fulani) in villages in Burkina Faso. The 2 groups are known to have distinct differences in their susceptibility and immune responses to malaria. We used RT-PCR and sequence-specific probes to detect and type RNA of the gametocyte-specific protein Pfs48/45. There were no differences in detection rates of asexual forms and gametocytes among the 2 groups, using PCR and RT-PCR, respectively. However, there were significant differences in densities of asexual forms and gametocytes, which were both higher among Mossi than Fulani. Both asexual forms and gametocyte densities were influenced by age and ethnicity. Multiple-clone infections with more than 1 gametocyte genotype were equally prevalent among Fulani and Mossi. These differences can most probably be attributed to genetic differences in malaria susceptibility in the 2 ethnic groups.

Keywords

Plasmodium falciparumgametocytesBurkina FasoFulaniMossi
Type
Research Article
Information
Parasitology , Volume 132 , Issue 5 , May 2006 , pp. 607 - 614
Copyright
2006 Cambridge University Press

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