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Glutathione-S-transferase of Trichinella spiralis regulates maturation and function of dendritic cells

Published online by Cambridge University Press:  30 August 2019

Xuemin Jin ,
Yong Yang ,
Xiaolei Liu ,
Haining Shi ,
Xuepeng Cai ,
Xuenong Luo ,
Mingyuan Liu  and
Xue Bai Open the ORCID record for Xue Bai [Opens in a new window]
Xuemin Jin
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Yong Yang
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Xiaolei Liu
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
Haining Shi
Affiliation:
Mucosal Immunology Laboratory, Pediatric Gastroenterology Unit, Massachusetts General Hospital, Boston, MA, USA
Xuepeng Cai
Affiliation:
China Institute of Veterinary Drugs Control, Beijing 100000, China State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China
Xuenong Luo
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China
Mingyuan Liu*
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225000, China.
Xue Bai*
Affiliation:
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China
*
Author for correspondence: Xue Bai, E-mail: baixue2008851001@jlu.edu.cn; Mingyuan Liu, E-mail: liumy@jlu.edu.cn
Author for correspondence: Xue Bai, E-mail: baixue2008851001@jlu.edu.cn; Mingyuan Liu, E-mail: liumy@jlu.edu.cn
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Abstract

Immunomodulation by molecules from Trichinella spiralis (T. spiralis) has been widely reported. Glutathione-S-transferase (GST) is a major immune-modulator of the family of detoxification enzymes. Dendritic cells (DCs) are an important target for the regulation of the immune response by T. spiralis. In this study, the recombinant GST of T. spiralis (rTs-GST) was expressed and purified. rTs-GST induced low CD40 expression and moderate CD80, CD86 and MHC-II expressions and inhibited the increase of CD40, CD80 and CD86 on DCs induced by LPS. We showed that rTs-GST decreased the LPS-induced elevated level of pro-inflammatory cytokines of DCs and enhanced the level of regulatory cytokines IL-10 and TGF-β. Furthermore, co-culture of DCs and CD4+ T cells demonstrated that rTs-GST-treated DCs suppressed the proliferation of OVA-specific CD4+ T cells and increased the population of regulatory T cells (Tregs). rTs-GST-treated DCs induced a higher level of IL-4, IL-10 and TGF-β, but inhibited the level of IFN-γ. This indicates that rTs-GST-pulsed DCs induce both Th2-type responses and Tregs. These findings contribute to the current understanding of the immunomodulation of Ts-GST on cellular response and immunomodulation of T. spiralis.

Keywords

Dendritic cellsglutathione-S-transferasesemi-maturationTh2 immune responseTregsTrichinella spiralis
Type
Research Article
Information
Parasitology , Volume 146 , Special Issue 14: BSP 2018 Autumn Symposium , December 2019 , pp. 1725 - 1732
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Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

*

These authors contributed equally to this study

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