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Host-mediated RNAi of a Notch-like receptor gene in Meloidogyne incognita induces nematode resistance

Published online by Cambridge University Press:  25 April 2018

Deshika Kohli ,
Parameswaran Chidambaranathan ,
J. Prasanth Tej Kumar ,
Ashish Kumar Singh ,
Anil Kumar ,
Anil Sirohi ,
K. Subramaniam ,
Ramamurthy Srinivasan ,
Navneeta Bharadvaja  and
Pradeep K. Jain Open the ORCID record for Pradeep K. Jain [Opens in a new window]
Deshika Kohli
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India Delhi Technological University, Shahbad Daulatpur, Delhi-110042, India
Parameswaran Chidambaranathan
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
J. Prasanth Tej Kumar
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
Ashish Kumar Singh
Affiliation:
Division of Nematology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India
Anil Kumar
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
Anil Sirohi
Affiliation:
Division of Nematology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India
K. Subramaniam
Affiliation:
Department of Biotechnology, Indian Institute of Technology, Madras, Chennai 600036, India
Ramamurthy Srinivasan
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
Navneeta Bharadvaja
Affiliation:
Delhi Technological University, Shahbad Daulatpur, Delhi-110042, India
Pradeep K. Jain*
Affiliation:
ICAR-NRC on Plant Biotechnology, Pusa Campus, New Delhi 110012, India
*
Author for correspondence: Pradeep K. Jain, E-mail: jainpmb@gmail.com
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Abstract

GLP-1 (abnormal germline proliferation) is a Notch-like receptor protein that plays an essential role in pharyngeal development. In this study, an orthologue of Caenorhabditis elegans glp-1 was identified in Meloidogyne incognita. A computational analysis revealed that the orthologue contained almost all the domains present in the C. elegans gene: specifically, the LIN-12/Notch repeat, the ankyrin repeat, a transmembrane domain and different ligand-binding motifs were present in orthologue, but the epidermal growth factor-like motif was not observed. An expression analysis showed differential expression of glp-1 throughout the life cycle of M. incognita, with relatively higher expression in the egg stage. To evaluate the silencing efficacy of Mi-glp-1, transgenic Arabidopsis plants carrying double-stranded RNA constructs of glp-1 were generated, and infection of these plants with M. incognita resulted in a 47–50% reduction in the numbers of galls, females and egg masses. Females obtained from the transgenic RNAi lines exhibited 40–60% reductions in the transcript levels of the targeted glp-1 gene compared with females isolated from the control plants. Second-generation juveniles (J2s), which were descendants of the infected females from the transgenic lines, showed aberrant phenotypes. These J2s exhibited a significant decrease in the overall distance from the stylet to the metacorpus region, and this effect was accompanied by disruption around the metacorporeal bulb of the pharynx. The present study suggests a role for this gene in organ (pharynx) development during embryogenesis in M. incognita and its potential use as a target in the management of nematode infestations in plants.

Keywords

Arabidopsisdouble-stranded RNA (dsRNA)glp-1 genein vivo RNAiMeloidogyne incognitapharynx development
Type
Research Article
Information
Parasitology , Volume 145 , Issue 14 , December 2018 , pp. 1896 - 1906
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Copyright
Copyright © Cambridge University Press 2018 

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