Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-10T16:19:32.885Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of field-caught Culicoides biting midges using matrix-assisted laser desorption/ionization time of flight mass spectrometry

Published online by Cambridge University Press:  19 October 2011

CHRISTIAN KAUFMANN ,
FRANCIS SCHAFFNER ,
DOMINIK ZIEGLER ,
VALENTIN PFLÜGER  and
ALEXANDER MATHIS
CHRISTIAN KAUFMANN
Affiliation:
Vector Entomology Unit, Institute of Parasitology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
FRANCIS SCHAFFNER
Affiliation:
Vector Entomology Unit, Institute of Parasitology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
DOMINIK ZIEGLER
Affiliation:
Mabritec SA, Riehen, Switzerland
VALENTIN PFLÜGER
Affiliation:
Mabritec SA, Riehen, Switzerland
ALEXANDER MATHIS*
Affiliation:
Vector Entomology Unit, Institute of Parasitology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
*
*Corresponding author: Vector Entomology Unit, Institute of Parasitology, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland. Tel: +41 44 635 85 01. Fax: +41 44 635 89 07. E-mail: alexander.mathis@uzh.ch
Get access Rights & Permissions [Opens in a new window]

Summary

Culicoides biting midges are of great importance as vectors of pathogens and elicitors of allergy. As an alternative for the identification of these tiny insects, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was evaluated. Protein mass fingerprints were determined for 4–5 field-caught reference (genetically confirmed) individuals of 12 Culicoides species from Switzerland, C. imicola from France, laboratory-reared C. nubeculosus and a non-biting midge. Reproducibility and accuracy of the database was tested in a validation study by analysing 108 mostly field-caught target Culicoides midges and 3 specimens from a non-target species. A reference database of biomarker mass sets containing between 24 and 38 masses for the different species could be established. Automated database-based identification was achieved for 101 of the 108 specimens. The remaining 7 midges required manual full comparison with the reference spectra yielding correct identification for 6 specimens and an ambiguous result for the seventh individual. Specimens of the non-target species did not yield identification. Protein profiling by MALDI-TOF, which is compatible with morphological and genetic identification of specimens, can be used as an alternative, quick and inexpensive tool to accurately identify Culicoides biting midges collected in the field.

Keywords

Culicoidesbiting midgeMALDI-TOF MSidentificationinsectvector
Type
Research Article
Information
Parasitology , Volume 139 , Issue 2 , February 2012 , pp. 248 - 258
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Boorman, J. (1974). The maintenance of laboratory colonies of Culicoides variipennis (Coq.), C. nubeculosus (Mg.) and C. riethi Kiff. (Diptera, Ceratopogonidae). Bulletin of Entomological Research 64, 371–377.CrossRefGoogle Scholar
Campbell, P. M. (2005). Species differentiation of insects and other multicellular organisms using matrix-assisted laser desorption/ionization time of flight mass spectrometry protein profiling. Systematic Entomology 30, 186–190.CrossRefGoogle Scholar
Caprioli, G., Cristalli, G., Ragazzi, E., Molin, L., Ricciutelli, M., Sagratini, G., Seraglia, R., Zuo, Y. and Vittori, S. (2010). A preliminary matrix-assisted laser desorption/ionization time-of-flight approach for the characterization of Italian lentil varieties. Rapid Communications in Mass Spectrometry 24, 2843–2848.CrossRefGoogle ScholarPubMed
Carpenter, S., Wilson, A. and Mellor, P. S. (2009). Culicoides and the emergence of bluetongue virus in northern Europe. Trends in Microbiology 17, 172–178.CrossRefGoogle ScholarPubMed
Cêtre-Sossah, C., Baldet, T., Delécolle, J. C., Mathieu, B., Perrin, A., Grillet, C. and Albina, E. (2004). Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in Africa and Europe. Veterinary Research 35, 325–337.CrossRefGoogle ScholarPubMed
Chaignat, V., Worwa, G., Scherrer, N., Hilbe, M., Ehrensperger, F., Batten, C., Cortyen, M., Hofmann, M. and Thuer, B. (2009). Toggenburg Orbivirus, a new bluetongue virus: initial detection, first observations in field and experimental infection of goats and sheep. Veterinary Microbiology 138, 11–19.CrossRefGoogle ScholarPubMed
Dallas, J. F., Cruickshank, R. H., Linton, Y. M., Nolan, D. V., Patakakis, M., Braverman, Y., Capela, R., Capela, M., Pena, I., Meiswinkel, R., Ortega, M. D., Baylis, M., Mellor, P. S. and Mordue (Luntz), A. J. (2003). Phylogenetic status and matrilineal structure of the biting midge, Culicoides imicola, in Portugal, Rhodes and Israel. Medical and Veterinary Entomology 17, 379–387.CrossRefGoogle ScholarPubMed
Delécolle, J.-C. (1985). Nouvelle contribution à l’étude systématique et iconographique des espèces du genre Culicoides (Diptera: Ceratopogonidae) du Nord-Est de la France. Université Louis Pasteur, Strasbourg, France.Google Scholar
Feltens, R., Gorner, R., Kalkhof, S., Groger-Arndt, H. and von Bergen, M. (2010). Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry. BMC Evolutionary Biology 10, 95.CrossRefGoogle ScholarPubMed
Goffredo, M. and Meiswinkel, R. (2004). Entomological surveillance of bluetongue in Italy: methods of capture, catch analysis and identification of Culicoides biting midges. Veterinaria Italiana 40, 260–265.Google ScholarPubMed
Hellberg, W., Mellor, P. S., Torsteinsdottir, S. and Marti, E. (2009). Insect bite hypersensitivity in the horse: comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus. Veterinary Immunology and Immunopathology 132, 62–67.CrossRefGoogle ScholarPubMed
Kaufmann, C., Schaffner, F. and Mathis, A. (2009). Monitoring of biting midges (Culicoides spp.), the potential vectors of the bluetongue virus the 12 climatic regions of Switzerland. Schweizer Archiv für Tierheilkunde 5, 205–513.CrossRefGoogle Scholar
Kaufmann, C., Ziegler, D., Schaffner, F., Carpenter, S., Pflüger, V. and Mathis, A. (2011). Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges. Medical & Veterinary Entomology 25, 32–38.CrossRefGoogle ScholarPubMed
Mazzeo, M. F., Giulio, B. D., Guerriero, G., Ciarcia, G., Malorni, A., Russo, G. L. and Siciliano, R. A. (2008). Fish authentication by MALDI-TOF mass spectrometry. Journal of Agricultural and Food Chemistry 56, 11071–11076.CrossRefGoogle ScholarPubMed
Meiswinkel, R., Baldet, T., de Deken, R., Takken, W., Delécolle, J.-C. and Mellor, P. S. (2008). The 2006 outbreak of bluetongue in northern Europe – the entomological perspective. Preventive Veterinary Medicine 87, 55–63.CrossRefGoogle ScholarPubMed
Meiswinkel, R., van Rijn, P., Leijs, P. and Goffredo, M. (2007). Potential new Culicoides vector of bluetongue virus in northern Europe. Veterinary Record 161, 564–565.CrossRefGoogle ScholarPubMed
Mellmann, A., Bimet, F., Bizet, C., Borovskaya, A. D., Drake, R. R., Eigner, U., Fahr, A. M., He, Y., Ilina, E. N., Kostrzewa, M., Maier, T., Mancinelli, L., Moussaoui, W., Prevost, G., Putignani, L., Seachord, C. L., Tang, Y. W. and Harmsen, D. (2009). High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. Journal of Clinical Microbiology 47, 3732–3734.CrossRefGoogle ScholarPubMed
Mellor, P. S., Boorman, J. and Baylis, M. (2000). Culicoides biting midges: their role as arbovirus vectors. Annual Review of Entomology 45, 307–340.CrossRefGoogle ScholarPubMed
Mellor, P. S. and Hamblin, C. (2004). African horse sickness. Veterinary Research 35, 445–466.CrossRefGoogle ScholarPubMed
Paweska, J. T., Venter, G. J. and Hamblin, C. (2005). A comparison of the susceptibility of Culicoides imicola and C. bolitinos to oral infection with eight serotypes of epizootic haemorrhagic disease virus. Medical and Veterinary Entomology 19, 200–207.CrossRefGoogle Scholar
Perera, M. R., Vargas, R. D. F. and Jones, M. G. K. (2005). Identification of aphid species using protein profiling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Entomologia Experimentalis et Applicata 117, 243–247.CrossRefGoogle Scholar
Santos, C., Paterson, R. R. M., Venancio, A. and Lima, N. (2010). Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Applied Microbiology 108, 375–385.CrossRefGoogle ScholarPubMed
Sauer, S. and Kliem, M. (2010). Mass spectrometry tools for the classification and identification of bacteria. Nature Reviews Microbiology 8, 74–82.CrossRefGoogle ScholarPubMed
Schwenkenbecher, J. M., Mordue Luntz, A. J. and Piertney, S. B. (2009). Phylogenetic analysis indicates that Culicoides dewulfi should not be considered part of the Culicoides obsoletus complex. Bulletin of Entomological Research 99, 371–375.CrossRefGoogle Scholar
Sloet van Oldruitenborgh-Oosterbaan, M. M., van Poppel, M., de Raat, I. J., van den Boom, R. and Savelkoul, H. F. (2009). Intradermal testing of horses with and without insect bite hypersensitivity in The Netherlands using an extract of native Culicoides species. Veterinary Dermatology 20, 607–614.CrossRefGoogle ScholarPubMed
Stephan, R., Ziegler, D., Pflüger, V., Vogel, G. and Lehner, A. (2010). Rapid genus- and species-specific identification of Cronobacter spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology 48, 2846–2851.CrossRefGoogle ScholarPubMed
Stevenson, L. G., Drake, S. K., Shea, Y. R., Zelazny, A. M. and Murray, P. R. (2010). Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species. Journal of Clinical Microbiology 48, 3482–3486.CrossRefGoogle ScholarPubMed
van Veen, S. Q., Claas, E. C. J. and Kuijper, E. J. (2010). High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. Journal of Clinical Microbiology 48, 900–907.CrossRefGoogle ScholarPubMed
Venter, G. J. and Meiswinkel, R. (1994). The virtual absence of Culicoides imicola (Diptera: Ceratopogonidae) in a light-trap survey of the colder, high-lying area of the eastern Orange Free State, South Africa, and implications for the transmission of arboviruses. Onderstepoort Journal of Veterinary Research 61, 327–340.Google Scholar
Wenk, C. E., Kaufmann, C., Schaffner, F. and Mathis, A. (2011). Molecular characterisation of Swiss Ceratopogonidae (Diptera) and evaluation of real-time PCR assays for the identification of Culicoides biting midges. Veterinary Parasitology (in Press).Google ScholarPubMed