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Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica

Published online by Cambridge University Press:  31 May 2013

J. HODGKINSON ,
K. CWIKLINSKI ,
N. J. BEESLEY ,
S. PATERSON  and
D. J. L. WILLIAMS
J. HODGKINSON*
Affiliation:
Veterinary Parasitology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK
K. CWIKLINSKI
Affiliation:
Veterinary Parasitology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK
N. J. BEESLEY
Affiliation:
Veterinary Parasitology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK
S. PATERSON
Affiliation:
Centre for Genomic Research, University of Liverpool, Liverpool L69 7ZB, UK
D. J. L. WILLIAMS
Affiliation:
Veterinary Parasitology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK
*
*Corresponding author. Veterinary Parasitology, Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK. Tel: +44 (0)151 7950223. E-mail: jhodgkin@liverpool.ac.uk
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Summary

Despite years of investigation into triclabendazole (TCBZ) resistance in Fasciola hepatica, the genetic mechanisms responsible remain unknown. Extensive analysis of multiple triclabendazole-susceptible and -resistant isolates using a combination of experimental in vivo and in vitro approaches has been carried out, yet few, if any, genes have been demonstrated experimentally to be associated with resistance phenotypes in the field. In this review we summarize the current understanding of TCBZ resistance from the approaches employed to date. We report the current genomic and genetic resources for F. hepatica that are available to facilitate novel functional genomics and genetic experiments for this parasite in the future. Finally, we describe our own non-biased approach to mapping the major genetic loci involved in conferring TCBZ resistance in F. hepatica.

Keywords

Fasciola hepaticaGalba truncatulaSNPsgenomemicrosatellitepopulation genetics
Type
Research Article
Information
Parasitology , Volume 140 , Issue 12: Genetic and genomic approaches to understanding drug resistance in parasites , October 2013 , pp. 1523 - 1533
Copyright
Copyright © Cambridge University Press 2013 

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