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Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4

Published online by Cambridge University Press:  17 October 2003

T. G. V. SILVEIRA ,
H. K. TAKAHASHI  and
A. H. STRAUS
T. G. V. SILVEIRA
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil Present address: Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, PR, 87020-900, Brasil.
H. K. TAKAHASHI
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
A. H. STRAUS
Affiliation:
Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
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Abstract

The immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. The antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. The reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogeneity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. The 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. In contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radio-immunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.

Keywords

Leishmania (Viannia) braziliensismonoclonal antibodyspecific antigensflagellum
Type
Research Article
Information
Parasitology , Volume 127 , Issue 5 , November 2003 , pp. 449 - 456
Copyright
2003 Cambridge University Press

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References

REFERENCES

BARRAL, A., PEDRAL-SAMPAIO, D., GRIMALDI, G. Jr., MOMEN, H., McMAHON-PRATT, D., JESUS, A. R., ALMEIDA, R., BADARÓ, R., BARRAL-NETTO, M., CARVALHO, E. M. & JOHNSON, W. D. Jr. (1991). Leishmaniasis in Bahia, Brazil: Evidence that Leishmania amazonensis produces a wide spectrum of clinical disease. American Journal of Tropical Medicine and Hygiene 44, 536–546.CrossRefGoogle Scholar
CONVIT, J., ULRICH, M., FERNÁNDEZ, C. T., TAPIA, F. J., CÁCERES-DITTMAR, G., CASTÉS, M. & RONDÓN, A. J. (1993). The clinical and immunological spectrum of American cutaneous leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 444–448.CrossRefGoogle Scholar
GRIMALDI, G. Jr., DAVID, J. R. & McMAHON-PRATT, D. (1987). Identification and distribution of New World Leishmania species characterized by serodeme analysis using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 36, 270–287.CrossRefGoogle Scholar
GRIMALDI, G. Jr. & McMAHON-PRATT, D. (1996). Monoclonal antibodies for the identification of New World Leishmania species. Memorias do Instituto Oswaldo Cruz 91, 37–42.CrossRefGoogle Scholar
GRIMALDI, G. Jr., MOMEN, H., NAIFF, R. D., McMAHON-PRATT, D. & BARRET, T. V. (1991). Characterization and classification of leishmanial parasites from humans, wild mammals, and sand flies in the Amazon region of Brasil. American Journal of Tropical Medicine and Hygiene 44, 645–661.CrossRefGoogle Scholar
HANHAM, C. A., SHAW, J. J. & LAINSON, R. (1991 a). Monoclonal antibodies that react with Leishmania (Viannia) naiffi. Journal of Parasitology 77, 27–38.Google Scholar
HANHAM, C. A., ZHAO, F., SHAW, J. J. & LAINSON, R. (1991 b). Monoclonal antibodies for the identification of New World Leishmania. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 220.Google Scholar
HERWALDT, B. L. (1999). Leishmaniasis. Lancet 354, 1191–1199.CrossRefGoogle Scholar
JAFFE, C. L., RACHAMIN, N. & SARFSTEIN, R. (1990). Characterization of two proteins from Leishmania donovani and their use for vaccination against visceral leishmaniasis. Journal of Immunology 144, 699–702.Google Scholar
KUTNER, S., PELLERIN, P. & BRÉNIÈRE, S. (1990). Identification of cytoplasmic antigens related to the major surface antigens of Leishmania braziliensis braziliensis and L. donovani chagasi. Parasitology Research 76, 185–191.CrossRefGoogle Scholar
KUTNER, S., PELLERIN, P., BRÉNIÈRE, S. F., DESJEUX, P. & DEDET, J. P. (1988). Identification and purification of a 72 kDa antigen of Leishmania braziliensis braziliensis present on the surface and in the cytoplasm of the promastigotes and its specific recognition by sera from mucocutaneous leishmaniasis patients. Memorias do Instituto Oswaldo Cruz 83, 431–433.CrossRefGoogle Scholar
LAEMMLI, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–685.CrossRefGoogle Scholar
LAINSON, R. & SHAW, J. J. (1987). Evolution, classification and geographical distribution. In The Leishmaniasis in Biology and Medicine (ed. Peters, W. E. & Killick-Kendrick, R. ), pp. 1–120. Academic Press, London.
LEGRAND, D., DESJEUX, P., PONT, F., BRÉNIÈRE, S. F., LEMESRE, J. P., SANTORO, F. & CAPRON, A. (1987). Identification of a major 72 kilodalton surface antigen in twelve isolates of Leishmania braziliensis braziliensis. Molecular and Biochemical Parasitology 24, 117–124.CrossRefGoogle Scholar
McMAHON-PRATT, D., BENNETT, E. & DAVID, J. R. (1982). Monoclonal antibodies that distinguish subspecies of Leishmania braziliensis. Journal of Immunology 129, 926–927.Google Scholar
McMAHON-PRATT, D., BENNETT, E. & JAFFE, C. L. (1984 a). Stage specific antigens of Leishmania. Federation Proceedings 43, 1628.Google Scholar
McMAHON-PRATT, D. & DAVID, J. R. (1981). Monoclonal antibodies that distinguish between New World species of Leishmania. Nature, London 291, 581–583.CrossRefGoogle Scholar
McMAHON-PRATT, D., JAFFE, C. L. & GRIMALDI, G. (1984 b). Application of monoclonal antibodies to the classification of Leishmania species. In Genes and Antigens of Parasites. A Laboratory Manual (ed. Morel, C. M.), pp. 133–135. Fundação Oswaldo Cruz, Rio de Janeiro.
MISLE, J. A., MÁRQUEZ, M. E. & HERNÁNDEZ, A. G. (1985). Solubilization and partial purification of a cell surface component of Leishmania braziliensis. Zeitschrift für Parasitenkunde 71, 419–428.CrossRefGoogle Scholar
MODABBER, F. (1993). Leishmaniasis. In Tropical Disease Research: Progress 1991–92: Eleventh Programme Report of the UNDP/WHO Special Programme for Research and Training in Tropical diseases (TDR), pp. 77–91. WHO, Geneva.
NAGAKURA, K., TACHIBANA, H., KANEDA, Y. & NAKAE, T. (1986). Leishmania braziliensis; localization of glycoproteins in promastigotes. Experimental Parasitology 61, 335–342.CrossRefGoogle Scholar
RODRÍGUEZ, N., HERNÁNDEZ, A. G. & MERINO, F. (1983). Effect of a purified excreted factor from Leishmania braziliensis on macrophage activity. International Archives on Allergy Application to Immunology 72, 206–210.CrossRefGoogle Scholar
SAMADY, J. A., JANNIGER, C. K. & SCHWARTZ, R. A. (1996). Cutaneous and mucocutaneous leishmaniasis. Pediatrics, Dermatology 57, 13–20.Google Scholar
SHAW, J. J., ISHIKAWA, E. A. Y. & LAINSON, R. (1989). A rapid and sensitive method for the identification of Leishmania with monoclonal antibodies using fluorescein-labelled avidin. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 783–784.CrossRefGoogle Scholar
SHAW, J. J., LAINSON, R., McMAHON-PRATT, D. & DAVID, J. R. (1986). Serodemes of the Leishmania braziliensis complex. In Leishmania Taxonomie et Phylogenèse. Applications Éco-épidémiologiques (ed. Rioux, J.), pp. 179–183. Colloque International, 2–6 July, 1984, Montpellier, IMEE.
SILVEIRA, T. G. V., SUZUKI, E., TAKAHASHI, H. K. & STRAUS, A. H. (2001). Inhibition of macrophage invasion by monoclonal antibodies specific to Leishmania (Viannia) braziliensis promastigotes and characterisation of their antigens. International Journal for Parasitology 31, 1451–1458.CrossRefGoogle Scholar
STRAUS, A. H., LEVERY, S. B., JASIULIONIS, M. G., SALYAN, M. E. K., STEELE, S. J., TRAVASSOS, L. R., HAKOMORI, S. & TAKAHASHI, H. K. (1993). Stage-specific glycosphingolipids from amastigote forms of Leishmania (L.) amazonensis. Journal of Biological Chemistry 268, 13723–13730.Google Scholar
STRAUS, A. H., FREYMULLER, E., TRAVASSOS, L. R. & TAKAHASHI, H. K. (1996). Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccididioicles brasiliensis with monoclonal antibodies. Journal of Medical and Veterinary Mycology 34, 181–186.CrossRefGoogle Scholar
SUZUKI, E., TOLEDO, M. S., TAKAHASHI, H. K. & STRAUS, A. H. (1997). A monoclonal antibody directed to terminal residue of β-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis: cross-reactivity with Leishmania major and Trypanosoma cruzi. Glycobiology 7, 463–468.CrossRefGoogle Scholar
TOWBIN, H., STAEHELIN, T. & GORDON, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4354.CrossRefGoogle Scholar