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In vitro influence of Theileria annulata on the functions of bovine dendritic cells for stimulation of T lymphocyte proliferation

Published online by Cambridge University Press:  10 September 2019

Muhammad Rashid Open the ORCID record for Muhammad Rashid [Opens in a new window] ,
Junlong Liu ,
Guiquan Guan ,
Jinming Wang Open the ORCID record for Jinming Wang [Opens in a new window] ,
Zhi Li ,
Muhammad Adeel Hassan ,
Muhammad Imran Rashid ,
Muhammad Uzair Mukhtar ,
Jianxun Luo  and
Hong Yin
Muhammad Rashid
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Junlong Liu*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Guiquan Guan
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Jinming Wang
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Zhi Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Muhammad Adeel Hassan
Affiliation:
Department of Parasitology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur63100, Pakistan
Muhammad Imran Rashid
Affiliation:
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore54200, Pakistan
Muhammad Uzair Mukhtar
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Jianxun Luo
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China
Hong Yin*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu730046, People's Republic of China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou225009, People's Republic of China
*
Author for correspondence: Junlon Liu, E-mail: liujunlong@caas.cn and Hong Yin, E-mail: yinhong@caas.cn
Author for correspondence: Junlon Liu, E-mail: liujunlong@caas.cn and Hong Yin, E-mail: yinhong@caas.cn
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Abstract

The present study was performed on antigen-presenting cells (APCs) of Theileria annulata transformed dendritic cells (TaDCs) and monocyte-derived dendritic cells (MoDCs) to compare differences in antigen presentation and stimulation of T lymphocyte proliferation. Antigen presentation for T lymphocyte proliferation was analysed by flow cytometry. Additionally, the level of mRNA transcription of small GTPases of the Rab family expressed in the TaDC cell line was analysed by quantitative real-time polymerase chain reaction (Q-RT-PCR). The endocytosis rate of TaDCs was significantly (P < 0.01) lower than in MoDCs. In contrast, when T lymphocytes were co-cultured with TaDC-APCs T cell proliferation was similar, while co-culture with MoDC-APC stimulated proliferation of CD4+ cells to a greater degree than CD8+ cells. However, the efficacy of TaDC-APCs to stimulate T lymphocytes dropped as the number of passages of TaDC-APC increased. Likewise, the transcription level of Rab family genes also significantly (P > 0.001) declined with progressive passages (>50) of the TaDC cell line. We conclude that initially the TaDC cell line efficiently presents antigen to stimulate T lymphocyte proliferation to produce a cellular immune response against the presented antigen.

Keywords

Antigen presenting cellsMHC moleculesRab family genessmall GTPaseT lymphocyte proliferation
Type
Research Article
Information
Parasitology , Volume 147 , Issue 1 , January 2020 , pp. 39 - 49
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Copyright
Copyright © Cambridge University Press 2019

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