Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-15T13:46:07.007Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix

Published online by Cambridge University Press:  06 April 2009

M. A. Fernando  and
J. Pasternak
M. A. Fernando
Affiliation:
Department of Pathology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N2G 2W2
J. Pasternak
Affiliation:
Department of Biology, Univeristy of Waterloo, Waterloo, ontario, Canada, N2L 3G1
Get access Rights & Permissions [Opens in a new window]

Extract

After infection with Eimeria necatrix, chick intestinal cells undergo a profound increase in size. Herien, we describe methods for isolating and purifying infected cells containing second-generation schizonts at Various times post-infection. Our purification procoedures depended upon three fundamental properties of infected cells: namely (1) large size, (2) resistance to shearing and (3) decreased susceptibility to osmotic shock in hypotonic solutions. The availability of simple techniques for isolating infected whole cells should prove valuable in elucidating various intracellular aspects of infection during schizogony.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 19 - 26
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beyer, T. V. & Shibalova, T. A. (1974). The increase of DNA quantity in the nuclei of chicken caecal cells harbouring second generation schizonts of Eimeria tenella. Parasitologia (Leningrad) 8, 449–55. (In Russion.)Google Scholar
Doran, D. J. (1973). Cultivation of coccidia in avian embryos and cell culture. In The Coccidia: Eimeria, Isospara, Toxoplasma and Related Genera (ed. Hammond, D. M. and Long, P. L.), pp. 183–252. Baltimore: University Park Press.Google Scholar
Fernando, M. A. & Stockdale, P. H. G. (1974). Fine structural changes associated with schizogony in Eimeria necatrix. Zeitschrift für Parasitenkunde 43, 105–14.CrossRefGoogle ScholarPubMed
Fernando, M. A., Pasternak, J., Barrell, R. & Stocdale, P. H. G. (1974). Induction of host nuclear DNA synthesis in coccidia-infected chicken intestinal cells. International Journal for Parasitology 4, 267–76.CrossRefGoogle ScholarPubMed
Hammond, D. M. (1971). The development and ecology of coccidia and related intracellular parasites. In Ecology and Physiology of Parasites, A Symposium (ed. Fallis, A. M.), pp.3–19. Toronto: University of Toronto Press.Google Scholar
Prescott, D. M., Rao, M. V. N., Evenson, D. P., Stone, G. E. & Thrasher, J. D. (1966). Isolation of single nuclei and mass preparations of nuclei from several cell types. In Methods in Cell Physiology, Vol. 2 (ed. Prescott, D. M.), pp. 131–42. New York: Academic Press.Google Scholar
Sampson, J. R. & Hammond, D. M. (1972). Fine structural aspects of development of Eimeria alabamensis schizonts in cell culture. Journal of Parasitology 58, 311–22.CrossRefGoogle Scholar