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Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice

Published online by Cambridge University Press:  30 January 2017

RÔMULO D. NOVAES ,
ELIZIÁRIA C. SANTOS ,
MARIA DO CARMO Q. FIALHO ,
WAGNER G. GONÇALVES ,
PRISCILA L. SEQUETTO ,
ANDRÉ TALVANI  and
REGGIANI V. GONÇALVES
RÔMULO D. NOVAES*
Affiliation:
Department Structural Biology, Federal University of Alfenas, MG 37130-000, Brazil
ELIZIÁRIA C. SANTOS
Affiliation:
School of Medicine, Federal University of Jequitinhonha and Mucuri Valleys, MG 39100-000, Brazil
MARIA DO CARMO Q. FIALHO
Affiliation:
Department of Morphology, Federal University of Amazonas, AM 69077-000, Brazil
WAGNER G. GONÇALVES
Affiliation:
Department of General Biology, Federal University of Viçosa, MG 36570-000, Brazil
PRISCILA L. SEQUETTO
Affiliation:
Department of Pharmaceutical Sciences – Health Area, Federal University of Juiz de Fora, MG 35020-220, Brazil
ANDRÉ TALVANI
Affiliation:
Department of Biological Sciences and NUPEB, Federal University of Ouro Preto, MG 34500-000, Brazil
REGGIANI V. GONÇALVES
Affiliation:
Department of Animal Biology, Federal University of Viçosa, MG 36570-000, Brazil
*
*Corresponding author. Department of Structural Biology, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, Alfenas, 37130-000 Minas Gerais, Brazil. E-mail: romuonovaes@yahoo.com.br
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Summary

We compared the relevance of ibuprofen, vitamins C and E to control oxidative/nitrosative stress and heart disease in mice infected by Trypanosoma cruzi. Swiss mice were randomized into five groups: control, uninfected; infected without treatment; and infected treated with vitamins C, E or ibuprofen. Animals were inoculated with 2000 trypomastigote forms of T. cruzi. After 20 days, infected mice presented reduced vitamin C and E tissue levels, high cytokines (interferon gamma, tumour necrosis factor-α, interleukin 10 and chemokine ligand 2), prostaglandin F2α (PGF2α) and nitric oxide (NO) cardiac production, intense myocarditis and reactive tissue damage, which was directly correlated with the intensity of the inflammatory infiltrate and the degree of pathological cardiac remodelling. Vitamins C and E supplementation were irrelevant to counteract reactive tissue damage and myocarditis in infected animals. Conversely, ibuprofen reduced tissue levels of cytokines, PGF2α and NO, as well as lipid and protein oxidation, antioxidant enzyme activity and the cardiac damage, without interfering with heart parasitism. Our results do not support the applicability of vitamin C and E supplementation in the management of acute Chagas cardiomyopathy. By controlling the inflammatory infiltrate, anti-inflammatory-based therapy proved to be a more rational strategy than a direct antioxidant therapy in attenuating oxidative/nitrosative stress and cardiac damage.

Keywords

Chagas diseasecardiovascular pathologyexperimental therapeuticsoxidative stress
Type
Research Article
Information
Parasitology , Volume 144 , Issue 7 , June 2017 , pp. 904 - 916
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Copyright
Copyright © Cambridge University Press 2017 

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