Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-18T17:45:16.621Z Has data issue: false hasContentIssue false
  • English
  • Français

Reaginic antibodies and immunity to Nippostrongylus brasiliensis in the rat

I. The effect of thymectomy, neonatal infections and splenectomy

Published online by Cambridge University Press:  06 April 2009

Bridget M. Ogilvie  and
Valerie E. Jones
Bridget M. Ogilvie
Affiliation:
National Institute for Medical Research, London, N. W. 7
Valerie E. Jones
Affiliation:
National Institute for Medical Research, London, N. W. 7
Get access Rights & Permissions [Opens in a new window]

Extract

Rats thymectomized at birth were infected with Nippostrongylus brasiliensis at 6 weeks of age. In some, but not all, rats egg production was greatly prolonged after the primary infection. Thymectomized rats were less resistant than controls to second infections.

In rats infected at birth the development of resistance was significantly delayed. Neonatally infected rats, when they were 6 weeks old, were less resistant to reinfection than adult controls. Rats infected three times in the neonatal period were more resistant to challenge at 6 weeks of age than rats infected once at birth, but less resistant than adult controls. The degree of immunity induced by a single neonatal infection was variable; some rats showed little or no resistance to reinfection 6 weeks later.

Antibody formation, measured by the occurrence of reagins, was altered in the same way by both neonatal thymectomy and neonatal infection. In the thymectomized (immunologically deficient) and newborn (immunologically immature) rats, reagin formation following initial infection was delayed; there was a reduced anamnestic response after challenge; mean titres were lower and more variable than in control animals; and in some rats circulating reagins were never detected.

In thymectomized rats the capacity to demonstrate anaphylaxis was as variable as in control rats, but in the few thymectomized rats in which egg production was prolonged, anaphylaxis was slight at a time when most intact rats had developed the capacity to show anaphylaxis. Rats infected at birth and adult rats infected at the same time were equally susceptible to systemic anaphylaxis 6 weeks later, although by this time there was a marked difference in the degree of resistance to the parasite developed by the two groups.

In adult rats splenectomy before, during or after initial infection had no effect either on the rate of development, or the degree of immunity induced by primary or secondary infections, or on antibody formation.

We would like to thank Miss W. Griffiths and A. J. Edwards for their willing technical assistance. This work was done whilst one of us (B.M.O.) was the holder of a Wellcome Fellowship of the Animal Health Trust.

Type
Research Article
Information
Parasitology , Volume 57 , Issue 2 , May 1967 , pp. 335 - 349
Copyright
Copyright © Cambridge University Press 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barth, E. E., Jarrett, W. F. H. & Urquhart, G. M. (1966). The induction of immunological unresponsiveness to Nippostrongylus brasiliensis in rats. Trans. R. Soc. trop. Med. Hyg. 60, 19.CrossRefGoogle Scholar
Ben-Efraim, S. & Leskowitz, S. (1966). Antigenicity of conjugates of arsanilic acid in inbred guinea pigs. Nature, Lond. 210, 1068–9.CrossRefGoogle ScholarPubMed
Binaghi, R. A. & Benacerraf, B. (1964). The production of anaphylactic antibody in the rat. J. Immunol. 92, 920–26.CrossRefGoogle ScholarPubMed
Binaghi, R. A., Oettgen, H. F. & Benacerraf, B. (1966). Anaphylactic antibody in the young rat. Int. Archs. Allergy appl. Immun. 29, 105–11.CrossRefGoogle ScholarPubMed
Good, R. A. & Papermaster, B. W. (1964). Ontogeny and phylogeny of adaptive immunity. Adv. Immunol. 4, 1–115.CrossRefGoogle Scholar
Janković, B. D., Waksman, B. H. & Arnason, B. G. (1962). Role of the thymus in immune reaction to rats. I. The immunologic response to bovine serum albumin (antibody formation, Arthus reactivity and delayed hypersensitivity) in rats thymectomised or splenectomised at various times after birth. J. exp. Med. 116, 159–75.Google Scholar
McDevitt, H. O. & Sela, M. (1965). Genetic control of the antibody response I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J. exp. Med. 122, 517–32.CrossRefGoogle ScholarPubMed
Mota, I. (1963). Biological characterisation of ‘mast cell sensitising’ antibodies. Life Sci. 1, 465.CrossRefGoogle Scholar
Ogilvie, B. M. (1964). Reagin-like antibodies in animals immune to helminth parasites. Nature, Lond. 204, 91–92.CrossRefGoogle ScholarPubMed
Ogilvie, B. M. (1965). Role of adult worms in immunity of rats to Nippostrongylus brasiliensis. Parasitology 55, 325–35.CrossRefGoogle ScholarPubMed
Ogilvie, B. M. (1967). Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis. Immunology 12, 113–32.Google ScholarPubMed
Ogilvie, B. M., Smithers, S. R. & Terry, R J. (1966). Reagin-like antibodies in experimental infections of Schistosoma mansoni and the passive transfer of resistance. Nature, Lond. 209, 1221–3.CrossRefGoogle ScholarPubMed
Ovary, Z. (1964). Passive cutaneous anaphylaxis. CIOMS. Symp. Immunol. Methods. (ed. Ackroyd, J. F.), p. 259. Oxford: Blackwell.Google Scholar
Stanworth, D. R. (1963). Reaginic antibodies. Adv. Immunol. 3, 181–260.CrossRefGoogle Scholar
Taliaferro, W. H. (1929). The Immunology of parasitic infection. New York: Century Co.Google Scholar
Wilson, R. J. M., Jones, V. E. & Leskowitz, S. (1967). Thymectomy and anaphylactic antibody in rats infected with Nippostrongylus brasiliensis. Nature, Lond. 213, 398–99.CrossRefGoogle ScholarPubMed