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Trypanosoma cruzi Discrete Typing Units in Chagas disease patients from endemic and non-endemic regions of Argentina

Published online by Cambridge University Press:  06 February 2012

C. I. CURA ,
R. H. LUCERO ,
M. BISIO ,
E. OSHIRO ,
L. B. FORMICHELLI ,
J. M. BURGOS ,
S. LEJONA ,
B. L. BRUSÉS ,
D. O. HERNÁNDEZ  and
G. V. SEVERINI
...Show all authors
C. I. CURA
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
R. H. LUCERO
Affiliation:
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina
M. BISIO
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
E. OSHIRO
Affiliation:
Centro Nacional de Diagnóstico e Investigación de Endemo-epidemias (CeNDIE), ANLIS Malbrán, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina
L. B. FORMICHELLI
Affiliation:
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina
J. M. BURGOS
Affiliation:
Departamento de Microbiología, Inmunología y Parasitología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Ciudad de Buenos Aires, Argentina
S. LEJONA
Affiliation:
Área de Biología Molecular, Centro de Especialidades Médicas Ambulatorias (CEMAR), San Luis 2020, 2000, Rosario, Santa Fe, Argentina
B. L. BRUSÉS
Affiliation:
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina
D. O. HERNÁNDEZ
Affiliation:
Consultorio de Chagas, Facultad de Medicina, Universidad Nacional del Nordeste, Moreno 1240, 3400, Corrientes, Argentina
G. V. SEVERINI
Affiliation:
Consultorio de Chagas, Facultad de Medicina, Universidad Nacional del Nordeste, Moreno 1240, 3400, Corrientes, Argentina
E. VELAZQUEZ
Affiliation:
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina
T. DUFFY
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
E. ANCHART
Affiliation:
Área de Biología Molecular, Centro de Especialidades Médicas Ambulatorias (CEMAR), San Luis 2020, 2000, Rosario, Santa Fe, Argentina
R. LATTES
Affiliation:
Instituto de Nefrología, Cabello 3889, 1425, Ciudad de Buenos Aires, Argentina
J. ALTCHEH
Affiliation:
Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Gallo 1330, 1425, Buenos Aires, Argentina
H. FREILIJ
Affiliation:
Servicio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Gallo 1330, 1425, Buenos Aires, Argentina
M. DIEZ
Affiliation:
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
C. NAGEL
Affiliation:
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
C. VIGLIANO
Affiliation:
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
L. FAVALORO
Affiliation:
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
R. R. FAVALORO
Affiliation:
Unidad de Trasplante Cardíaco, Hospital Universitario Fundación Favaloro, Av. Belgrano 1746, 1093, Ciudad de Buenos Aires, Argentina
D. E. MERINO
Affiliation:
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Av. Las Heras 727, 3500, Resistencia, Chaco, Argentina
S. SOSA-ESTANI
Affiliation:
Centro Nacional de Diagnóstico e Investigación de Endemo-epidemias (CeNDIE), ANLIS Malbrán, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina Instituto Nacional de Parasitología Dr. Mario Fatala Chaben, Av. Paseo Colón 568, 1063, Ciudad de Buenos Aires, Argentina
A. G. SCHIJMAN*
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 2do piso, 1428, Ciudad de Buenos Aires, Argentina
*
*Corresponding author: Vuelta de Obligado 2490, Buenos Aires 1428, Argentina. Tel: +54 11 47832871. Fax: +54 11 47868576. E-mail: schijman@dna.uba.ar
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Summary

Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis of Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. cruzi DTUs in bloodstream and tissue samples of Argentinean patients with Chagas disease. PCR-based strategies allowed DTU identification in 256 clinical samples from 239 Argentinean patients. Tc V prevailed in blood from both asymptomatic and symptomatic cases and Tc I was more frequent in bloodstream, cardiac tissues and chagoma samples from immunosuppressed patients. Tc II and VI were identified in a minority of cases, while Tc III and Tc IV were not detected in the studied population. Interestingly, Tc I and Tc II/VI sequences were amplified from the same skin biopsy slice from a kidney transplant patient suffering Chagas disease reactivation. Further data also revealed the occurrence of mixed DTU populations in the human chronic infection. In conclusion, our findings provide evidence of the complexity of the dynamics of T. cruzi diversity in the natural history of human Chagas disease and allege the pathogenic role of DTUs I, II, V and VI in the studied population.

Keywords

Trypanosoma cruziPCRDiscrete Typing UnitChagas disease
Type
Research Article
Information
Parasitology , Volume 139 , Issue 4 , April 2012 , pp. 516 - 521
Copyright
Copyright Published by Cambridge University Press 2012

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