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Ultrastructural changes to the tegumental system and the gastrodermal cells in adult Fasciola hepatica following in vivo treatment with the experimental fasciolicide, compound alpha

Published online by Cambridge University Press:  16 April 2009

M. McCONVILLE ,
G. P. BRENNAN ,
A. FLANAGAN ,
H. W. J. EDGAR ,
R. CASTILLO ,
A. HERNÁNDEZ-CAMPOS  and
I. FAIRWEATHER
M. McCONVILLE
Affiliation:
Parasite Proteomics and Therapeutics Research Group, Queens University Belfast
G. P. BRENNAN
Affiliation:
Parasite Proteomics and Therapeutics Research Group, Queens University Belfast
A. FLANAGAN
Affiliation:
Parasite Proteomics and Therapeutics Research Group, Queens University Belfast
H. W. J. EDGAR
Affiliation:
Veterinary Sciences Division, Agri-Food Biosciences Institute (AFBI), Stormont, Belfast
R. CASTILLO
Affiliation:
Facultads de Quimica y Medicina Veterinaria, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City
A. HERNÁNDEZ-CAMPOS
Affiliation:
Facultads de Quimica y Medicina Veterinaria, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City
I. FAIRWEATHER*
Affiliation:
Parasite Proteomics and Therapeutics Research Group, Queens University Belfast
*
*Corresponding author: Parasite Proteomics and Therapeutics Research Group, School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland. Tel: +44 28 9097 2298. Fax: +44 28 9097 5877. E-mail: i.fairweather@qub.ac.uk
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Summary

Sheep infected with the triclabendazole-susceptible, Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48 and 72 h post-treatment (p.t.). Ultrastructural changes to the flukes were assessed using transmission electron microscopy (TEM), with a view to gathering information on the mechanism(s) of action for compound alpha and on the possible route of its entry into F. hepatica. The tegumental syncytium was more severely affected than the gut at all time-points p.t. with compound alpha, suggesting a predominantly trans-tegumental route of uptake. Disruption to the tegumental system became increasingly severe over time. A stress response was observed at 24 h p.t. and took the form of blebbing and increases in the production and transport of secretory bodies. By 72 h p.t., extensive tegumental loss and degeneration of the tegumental cell bodies had occurred. Degeneration of subtegumental tissues and internal flooding were also observed. Changes in the gastrodermal cells were slow to develop: reduced secretory activity was evident at 72 h p.t.. There was progressive disruption to the somatic muscle layers, with disorganization of the muscle blocks and loss of muscle fibres.

Keywords

Fasciola hepaticaliver flukecompound alphatransmission electron microscopy
Type
Research Article
Information
Parasitology , Volume 136 , Issue 6 , May 2009 , pp. 665 - 680
Copyright
Copyright © Cambridge University Press 2009

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