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Amoebicidal activity of Cassia angustifolia extract and its effect on Acanthamoeba triangularis autophagy-related gene expression at the transcriptional level

Published online by Cambridge University Press:  10 May 2021

Rachasak Boonhok ,
Suthinee Sangkanu ,
Roghayeh Norouzi ,
Abolghasem Siyadatpanah ,
Farzaneh Mirzaei ,
Watcharapong Mitsuwan ,
Nurdina Charong ,
Sueptrakool Wisessombat ,
Maria de Lourdes Pereira  and
Mohammed Rahmatullah
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Rachasak Boonhok
Affiliation:
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat80160, Thailand
Suthinee Sangkanu
Affiliation:
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team) and World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat80160, Thailand
Roghayeh Norouzi
Affiliation:
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz51664, Iran
Abolghasem Siyadatpanah
Affiliation:
Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand9717853577, Iran
Farzaneh Mirzaei
Affiliation:
Department Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd14188-15971, Iran
Watcharapong Mitsuwan
Affiliation:
School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team) and World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat80160, Thailand Akkhraratchakumari Veterinary College, and Research Center of Excellence in Innovation of Essential Oil, Walailak University, Nakhon Si Thammarat80160, Thailand
Nurdina Charong
Affiliation:
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat80160, Thailand
Sueptrakool Wisessombat
Affiliation:
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat80160, Thailand
Maria de Lourdes Pereira
Affiliation:
Department of Medical Sciences, CICECO-Aveiro Institute of Materials, University of Aveiro, Aveiro3810-193, Portugal
Mohammed Rahmatullah
Affiliation:
Department of Biotechnology and Genetic Engineering, University of Development Alternative Lalmatia, Dhaka1209, Bangladesh
Polrat Wilairatana*
Affiliation:
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok10400, Thailand
Christophe Wiart
Affiliation:
School of Pharmacy, University of Nottingham Malaysia Campus, Selangor43500, Malaysia
Hazel Anne Tabo
Affiliation:
Biological Sciences Department, College of Science and Computer Studies, De La Salle University-Dasmarinas, Cavite4115, Philippines
Karma G. Dolma
Affiliation:
Department of Microbiology, Sikkim Manipal Institute of Medical Sciences (SMIMS), Gangtok, Sikkim737102, India
Veeranoot Nissapatorn*
Affiliation:
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat80160, Thailand School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team) and World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat80160, Thailand
*
Authors for correspondence: Polrat Wilairatana, E-mail: polrat.wil@mahidol.ac.th; Veeranoot Nissapatorn, E-mail: nissapat@gmail.com
Authors for correspondence: Polrat Wilairatana, E-mail: polrat.wil@mahidol.ac.th; Veeranoot Nissapatorn, E-mail: nissapat@gmail.com
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Abstract

Cassia angustifolia Vahl. plant is used for many therapeutic purposes, for example, in people with constipation, skin diseases, including helminthic and parasitic infections. In our study, we demonstrated an amoebicidal activity of C. angustifolia extract against Acanthamoeba triangularis trophozoite at a micromolar level. Scanning electron microscopy (SEM) images displayed morphological changes in the Acanthamoeba trophozoite, which included the formation of pores in cell membrane and the membrane rupture. In addition to the amoebicidal activity, effects of the extract on surviving trophozoites were observed, which included cyst formation and vacuolization by a microscope and transcriptional expression of Acanthamoeba autophagy in response to the stress by quantitative polymerase chain reaction. Our data showed that the surviving trophozoites were not transformed into cysts and the trophozoite number with enlarged vacuole was not significantly different from that of untreated control. Molecular analysis data demonstrated that the mRNA expression of AcATG genes was slightly changed. Interestingly, AcATG16 decreased significantly at 12 h post treatment, which may indicate a transcriptional regulation by the extract or a balance of intracellular signalling pathways in response to the stress, whereas AcATG3 and AcATG8b remained unchanged. Altogether, these data reveal the anti-Acanthamoeba activity of C. angustifolia extract and the autophagic response in the surviving trophozoites under the plant extract pressure, along with data on the formation of cysts. These represent a promising plant for future drug development. However, further isolation and purification of an active compound and cytotoxicity against human cells are needed, including a study on the autophagic response at the protein level.

Keywords

Acanthamoeba triangularisautophagyCassia angustifoliaencystation
Type
Research Article
Information
Parasitology , Volume 148 , Issue 9 , August 2021 , pp. 1074 - 1082
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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