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Amount of cholesterol in host membrane affects erythrocyte invasion and replication by Babesia bovis

Published online by Cambridge University Press:  06 December 2006

K. OKUBO ,
N. YOKOYAMA ,
N. TAKABATAKE ,
M. OKAMURA  and
I. IGARASHI
K. OKUBO
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
N. YOKOYAMA*
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
N. TAKABATAKE
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
M. OKAMURA
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
I. IGARASHI
Affiliation:
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
*
*Corresponding author: Tel: +81 155 49 5649. Fax: +81 155 49 5643. E-mail: yokoyama@obihiro.ac.jp
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Summary

Cholesterol is a major component of the erythrocyte membrane. In the present study, we investigated the effects of cholesterol reduction in host bovine erythrocytes (RBC) on the growth of Babesia bovis, a major bovine haemoprotozoon. An in vitro growth assay with bovine RBC that had been prepared by pre-treatment with a cholesterol depletion agent (methyl-β-cyclodextrin, MCD) showed that the culture with 5 mm MCD-treated RBC inhibited the growth of B. bovis significantly as compared with that with the control RBC. In further experiments, the treatment with 5 mm MCD was proved to suppress both activities of the parasite, erythrocyte invasion and replication within the infected RBC. In contrast, a slight reduction in the membrane cholesterol by 1 mm MCD treatment promoted both their growth and erythrocyte invasion activity. These results indicate that erythrocyte invasion and replication by B. bovis are affected by the amount of cholesterol in the host erythrocyte membrane.

Keywords

Babesia bovischolesterolerythrocytes
Type
Research Article
Information
Parasitology , Volume 134 , Issue 5 , May 2006 , pp. 625 - 630
Copyright
Copyright © Cambridge University Press 2006

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References

REFERENCES

Bork, S., Okamura, M., Matsuo, T., Kumar, S., Yokoyama, N. and Igarashi, I. (2005). Host serum modifies the drug susceptibility of Babesia bovis in vitro. Parasitology 130, 489–492.Google Scholar
Brown, W. C. and Palmer, G. H. (1999). Designing blood-stage vaccines against Babesia bovis and B. bigemina. Parasitology Today 15, 275–281.CrossRefGoogle ScholarPubMed
Christian, A. E., Haynes, M. P., Phillips, M. C. and Rothblat, G. H. (1997). Use of cyclodextrins for manipulating cellular cholesterol content. Journal of Lipid Research 38, 2264–2272.CrossRefGoogle ScholarPubMed
Coppens, I. and Joiner, K. A. (2003). Host but not parasite cholesterol controls Toxoplasma cell entry by modulating organelle discharge. Molecular Biology of the Cell 14, 3804–3820.CrossRefGoogle Scholar
Coppens, I., Sinai, A. P. and Joiner, K. A. (2000). Toxoplasma gondii exploits host low-density lipoprotein receptor-mediated endocytosis for cholesterol acquisition. The Journal of Cell Biology 149, 167–180.Google Scholar
Deuticke, B. and Ruska, C. (1976). Changes of nonelectrolyte permeability in cholesterol-loaded erythrocytes. Biochimica et Biophysica Acta 433, 638–653.CrossRefGoogle ScholarPubMed
Franssen, F. F., Gaffar, F. R., Yatsuda, A. P. and De Vries, E. (2003). Characterization of erythrocyte invasion by Babesia bovis merozoites efficiently released from their host cell after high-voltage pulsing. Microbes and Infection 5, 365–372.CrossRefGoogle ScholarPubMed
Homer, M. J., Aguilar-Delfin, I., Telford, S. R., and Persing, D. H. (2000). Babesiosis. Clinical Microbiology Review 13, 451–469.CrossRefGoogle ScholarPubMed
Igarashi, I., Aikawa, M. and Kreier, J. P. (1988). Host cell-parasite interactions in babesiosis. In Babesiosis of Domestic Animals and Man (ed. Ristic, M.), pp. 53–70. CRC Press, Florida.Google Scholar
Kuttler, K. L. (1988). Worldwide impact of babesiosis. In Babesiosis of Domestic Animals and Man (ed. Ristic, M.), pp. 1–22. CRC Press, Florida.Google Scholar
Lauer, S., Vanwye, J., Harrison, T., McManus, H., Samuel, B. U., Hiller, N. L., Mohandas, N. and Haldar, K. (2000). Vacuolar uptake of host components, and a role for cholesterol and sphingomyelin in malarial infection. The EMBO Journal 19, 3556–3564.Google Scholar
Niu, S. L. and Litman, B. J. (2002). Determination of membrane cholesterol partition coefficient using a lipid vesicle-cyclodextrin binary system: Effect of phospholipid acyl chain unsaturation and headgroup composition. Biophysical Journal 83, 3408–3415.CrossRefGoogle ScholarPubMed
Ohtani, Y., Irie, T., Uekama, K., Fukunaga, K. and Pitha, J. (1989). Differential effects of alpha-, beta-, and gamma-cyclodextrins on human erythrocytes. European Journal of Biochemistry 186, 17–22.Google Scholar
Okamura, M., Yokoyama, N., Wickramathilaka, N. P., Takabatake, N., Ikehara, Y. and Igarashi, I. (2005). Babesia caballi and Babesia equi: Implications of host sialic acids in erythrocyte infection. Experimental Parasitology 110, 406–411.CrossRefGoogle ScholarPubMed
Okubo, K., Wilawan, P., Bork, S., Okamura, M., Yokoyama, N. and Igarashi, I. (2006). Calcium-ion is involved in erythrocyte invasion by equine Babesia parasites. Parasitology 133, 289–294.Google Scholar
Samuel, B. U., Mohandas, N., Harrison, T., McManus, H., Rosse, W., Reid, M. and Haldar, K. (2001). The role of cholesterol and glycosylphosphatidylinositol-anchored proteins of erythrocyte rafts in regulating raft protein content and malarial infection. The Journal of Biological Chemistry 276, 29319–29329. DOI: 10.1074/jbc.M101268200Google Scholar
Shiga, T., Maeda, N. and Kon, K. (1990). Erythrocyte rheology. Critical Reviews in Oncology/Hematology 10, 9–48.Google Scholar
Simons, K. and Ikonen, E. (1997). Functional rafts in cell membranes. Nature, London 387, 569–572.CrossRefGoogle ScholarPubMed
Yawata, Y. (2003). Cell Membrane, Wiley-Vch Verlag, Weinheim Germany.CrossRefGoogle Scholar
Yokoyama, N., Kumar, S., Bork, S., Okamura, M. and Igarashi, I. (2005). Molecular approaches for the development of babesial vaccines. In Asian Parasitology, Toxoplasmosis and Babesiosis in Asia (ed. Yano, A., Nam, H- W., Anuar, K. A. and Shen, J.), pp. 214–223. AAA Committee, The Federation of Asian Parasitologists, Chiba, Japan.Google Scholar
Yokoyama, N., Okamura, M. and Igarashi, I. (2006). Erythrocyte invasion by Babesia parasites: Current advances in the elucidation of the molecular interactions between the protozoan ligands and host receptors in the invasion stage. Veterinary Parasitology 138, 22–32.CrossRefGoogle ScholarPubMed