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An artificial feeding technique for Glossina

Published online by Cambridge University Press:  06 April 2009

S. K. Moloo
S. K. Moloo
Affiliation:
East African Trypanosomiasis Research Organization, Tororo, Uganda
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Extract

An apparatus for the artificial feeding of tsetse has been described. Fresh defibrinated blood at 36 °C is circulated with a peristaltic pump through feeding capsules. The efficiency of the method has been compared with that of the Cockings' method and with the live host feeding. The flies exposed to feeding on this apparatus showed significantly higher feeding frequency than those allowed to feed on the Cockings' apparatus. Compared with the live host feeding, the feeding frequency for circulating blood method was slightly lower but the difference was not significant. Once the fly is induced to feed, it would in most cases complete engorgement unless disturbed. The rate of crop emptying in flies fed by the Cockings' method was abnormally slower as compared to those fed on the circulating blood. The present study indicates that for the successful artificial feeding of tsetse, it is essential to circulate the blood and regulate the temperature of the medium and the feeding surface to approximately 36 °C.

I wish to thank Mr S. B. Kutuza for technical help, and the Director of E.A.T.R.O. for permission to publish this work.

Type
Research Article
Information
Parasitology , Volume 63 , Issue 3 , December 1971 , pp. 507 - 512
Copyright
Copyright © Cambridge University Press 1971

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References

Cookings, K. L. (1960). Laboratory maintenance of Glossina pallidipes. East African Trypanosomiasis Research Organization Report 1959, pp. 13–18.Google Scholar
De Azevedo, J. F., Pinhao, R. da C. & Dos Sandos, A. M. T. (1968). Artificial breeding of tsetse fly. I – Factors connected with the feeding membrane and blood meals type. Anais Da Escola Nacional De Saude Publica E De Medicina Tropical 2, 11–17.Google ScholarPubMed
Galun, R. & Margalit, J. (1969). Adenine nucleotides as feeding stimulants of the tsetse fly Glossina austeni Newst. Nature, London 222, 583–4.CrossRefGoogle ScholarPubMed
Harley, J. M. B. & Cockings, K. L. (1968). A colony of G. pallidipes. East African Trypanosomiasis Research Organization Report 1967, pp. 36–6.Google Scholar
Kimber, C. D. & Harley, J. M. B. (1965). A fibrin membrane for feeding Glossina. East African Trypanosomiasis Research Organization Report 1963–1964, p. 45.Google Scholar
Langley, P. A. (1971). Rearing of tsetse flies in the absence of a living host. Transactions of the Royal Society of Tropical Medicine and Hygiene (In Press).CrossRefGoogle Scholar
Langley, P. A. & Maly, H. (1969). A membrane-feeding technique for tsetse flies. Nature, London 221, 855–6.CrossRefGoogle ScholarPubMed
Lumsden, W. H. R. (1964). Changing patterns of trypanosomiasis research in East Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene 58, 97–127.CrossRefGoogle ScholarPubMed
Lumsden, W. H. R. & Saunders, D. S. (1966). Tsetse flies. In Insect Colonization and Mass Production. New York: Academic Press Inc.Google Scholar
Moloo, S. K. & Kutuza, S. B. (1970). Feeding and crop emptying in Glossina brevipalpis. Acta Tropica 27, 356–77.Google ScholarPubMed
Nash, T. A. M., Jordan, A. M. & Boyle, J. A. (1966). The large scale rearing of G. austeni in the laboratory. Annales of Tropical Medicine and Parasitology 60, 469–81.CrossRefGoogle Scholar
Nelson, W. A. (1955). Artificial feeding of certain ectoparasites through membranes. Journal of Parasitology 41, 635–6.CrossRefGoogle Scholar
Rogers, A. & Kenyanjui, E. N. (1969). A colony of G. pallidipes. East African Trypanosomiasis Research Organization Report 1968, pp. 44–9.Google Scholar
Southon, H. A. W. & Cookings, K. L. (1963). Laboratory maintenance of Glossina. East African Trypanosomiasis Research Organization Report 1962–1963, pp. 31–33.Google Scholar
Tarshis, I. B. (1958). Feeding techniques for bloodsucking arthropods. Proceedings 10th International Congress of Entomology 3(1956), 767–84.Google Scholar