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An association between Schistosoma mansoni worms and an enzymatically-active protease/peptidase in mouse blood

Published online by Cambridge University Press:  22 January 2008

H. Y. DARANI  and
M. J. DOENHOFF
H. Y. DARANI*
Affiliation:
School of Biological Sciences, University of Bangor, Bangor, Gwynedd LL57 2UW, UK
M. J. DOENHOFF
Affiliation:
School of Biological Sciences, University of Bangor, Bangor, Gwynedd LL57 2UW, UK
*
*Corresponding author: Department of Parasitology, Cell and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran. Tel: 0098 381 2222751. Fax: 0098 381 2221669. E-mail: H_yousofi@yahoo.com
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Summary

An enzyme found previously in extracts of adult Schistosoma mansoni worms, that hydrolysed the chromogenic substrate N-acetyl-DL-phenylalanine β-naphthyl-ester, has here been further investigated and characterized. Evidence that the molecule found in the parasite was antigenically and enzymatically homologous with a constituent of normal mouse plasma has been consolidated using a monospecific serum in immunoelectrophoresis and Western immunoblotting. The molecular size of the enzyme was found to be approximately 70 kDa and it was inhibited by a serine protease inhibitor, but not by inhibitors of other classes of protease. The enzymatic activity found in normal mouse serum was also found in normal rat serum, but not in sera from several other mammalian species.

Keywords

schistosomeSchistosoma mansoniproteaseesterasenaphthylamidasemouserathostantigenserumimmune evasion
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 4 , April 2008 , pp. 467 - 472
Copyright
Copyright © Cambridge University Press 2008

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