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An improved method of inoculating plants with virus for the study of local lesions

Published online by Cambridge University Press:  06 April 2009

W. D. MacClement
Affiliation:
From the Molteno Institute and Potato Virus Research Station, University of Cambridge

Extract

An account is given of an investigation of the causes of error in estimating virus concentrations by the local lesion method. The irregularities were found to be due to:

(1) Variations in the response of (a) whole plants; (b) individual leaves.

(2) Variations in the volume of liquid used.

(3) Variations in the probability of the production of local lesions.

(4) Variations in the percentage of leaf hairs broken during the inoculation process.

An apparatus is described, the use of which reduces these irregularities to a minimum.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1937

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References

REFERENCES

Bald, J. G. (1935). Nature, Lond., 135, 996.Google Scholar
Holmes, F. O. (1929). Bot. Gaz. 87, 5663.Google Scholar
Samuel, G. (1931). Ann. appl. Biol. 18, 494507.CrossRefGoogle Scholar
Samuel, G. & Bald, J. G. (1933). Ann. appl. Biol. 20, 7099.Google Scholar
Youden, W. J. & Beale, Helen Purdy (1934). Contr. Boyce Thompson Inst. 6, 437–54.Google Scholar