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Anthelmintic activity of some Mediterranean browse plants against parasitic nematodes

Published online by Cambridge University Press:  07 December 2009

F. MANOLARAKI ,
S. SOTIRAKI ,
A. STEFANAKIS ,
V. SKAMPARDONIS ,
M. VOLANIS  and
H. HOSTE
F. MANOLARAKI
Affiliation:
NAGREF-VRI NAGREF Campus, Thermi57001PO Box 60272Thessaloniki, Greece UMR 1225 INRA/ENVT. Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076ToulouseCedex, France
S. SOTIRAKI
Affiliation:
NAGREF-VRI NAGREF Campus, Thermi57001PO Box 60272Thessaloniki, Greece
A. STEFANAKIS
Affiliation:
NAGREF-Subtropical Plant and Olive Tree Institute of Chania, Argokepion73100, ChaniaCreta, Greece
V. SKAMPARDONIS
Affiliation:
NAGREF-VRI NAGREF Campus, Thermi57001PO Box 60272Thessaloniki, Greece
M. VOLANIS
Affiliation:
NAGREF-Subtropical Plant and Olive Tree Institute of Chania, Argokepion73100, ChaniaCreta, Greece
H. HOSTE*
Affiliation:
UMR 1225 INRA/ENVT. Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076ToulouseCedex, France
*
*Corresponding author: UMR 1225 INRA/ENVT. Ecole Nationale Vétérinaire de Toulouse – 23 Chemin des Capelles, 31076ToulouseCedex,France. Tel: +33 5 61 19 38 75. Fax: +33 5 61 19 32 43. E-mail: h.hoste@envt.fr
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Summary

The anthelmintic properties of tannin-rich plants are being explored as an alternative to chemical drugs. Most data have been acquired on legume forages, but only few on browse plants. The present study aimed to (i) screen the in vitro effects of extracts from 7 Mediterranean plants on Haemonchus contortus, (ii) verify the role of tannins using an inhibitor, polyvinyl polypyrrolidone (PVPP) and (iii) verify the in vivo effects of extracts from 4 plants. Significant inhibition was shown in vitro using a larval migration inhibition (LMI) assay for all extracts except that from Olea europaea var. koroneiki. After adding PVPP, the LMI values were restored to control levels for all plants except Pistacia lentiscus and Ceratonia siliqua, confirming a role for tannins in the activity. In the in vivo experiment, 48 lambs composed 6 groups, depending on diet. On Day 0, groups G1–G5 received H. contortus and Trichostrongylus colubriformis larvae and G6 remained uninfected. The various diets were distributed from Days 14 to 45: P. lentiscus (G1), Quercus coccifera (G2), C. siliqua (G3), Onobrychis viciifolia (G4), or Medicago sativa for the 2 control groups (G5, G6). Egg excretion, packed cell volumes (PCVs) and inorganic phosphate were measured weekly throughout the entire experimental period. At slaughter, the worms were enumerated and their fecundity assessed. Consumption of the 4 browser plants did not provoke differences in pathophysiological measurements but there were significant decreases in egg excretion, mainly explained by significant decreases in worm fecundity for both species, without any statistical difference in worm numbers.

Keywords

nematodestanninsbrowse plantsnatural anthelminticsheep
Type
Research Article
Information
Parasitology , Volume 137 , Issue 4 , April 2010 , pp. 685 - 696
Copyright
Copyright © Cambridge University Press 2009

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