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Anti-SAG1 peptide antibodies inhibit the penetration of Toxoplasma gondii tachyzoites into enterocyte cell lines

Published online by Cambridge University Press:  28 November 2001

F. VELGE-ROUSSEL ,
I. DIMIER-POISSON ,
D. BUZONI-GATEL  and
D. BOUT
F. VELGE-ROUSSEL
Affiliation:
UMR UNIVERSITE-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France
I. DIMIER-POISSON
Affiliation:
UMR UNIVERSITE-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France
D. BUZONI-GATEL
Affiliation:
UMR UNIVERSITE-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France
D. BOUT
Affiliation:
UMR UNIVERSITE-INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France
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Abstract

The initial attachment of Toxoplasma tachyzoites to the target host cell is an important event in the life-cycle of the parasite and a critical stage in infection. Previous studies have shown that polyclonal antibodies directed against the major surface antigen of Toxoplasma gondii (SAG1) inhibit the infection of enterocyte cell lines. Here, we demonstrate that antibodies raised against a central peptide (V41T) of SAG1 and the SAG1 protein itself are able to inhibit the infection of various cell lines by the tachyzoites. Antibodies directed against SAG1 peptides were used to define a site on the SAG1 antigen that interacts with the host cell. The epitope carried by V41T was identified on the tachyzoite surface by immunofluorescence. The peptide sequence seems to be conserved in all the members of the SAG1 Related Sequence family (SRS). Using undifferentiated and differentiated Caco-2 cells, we found that tachyzoites enter preferentially via the basolateral side of the cell. These findings highlight the role of the SRS family members in the mediation of host cell invasion.

Keywords

Toxoplasma gondiiSAG1invasion
Type
Research Article
Information
Parasitology , Volume 123 , Issue 3 , March 2001 , pp. 225 - 233
Copyright
© 2002 Cambridge University Press

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