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Assignment of Vairimorpha leptinotarsae comb. nov. on the basis of molecular characterization of Nosema leptinotarsae Lipa, 1968 (Microsporidia: Nosematidae)

Published online by Cambridge University Press:  27 April 2020

Çağrı Bekircan Open the ORCID record for Çağrı Bekircan [Opens in a new window]
Çağrı Bekircan*
Affiliation:
Department of Veterinary, Sarayönü Vocational School, Selçuk University, 42430, Konya, Turkey
*
Author for correspondence: Çağrı Bekircan, E-mail: cagribekircan@hotmail.com
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Abstract

Nosema leptinotarsae Lipa, 1968 is a microsporidian pathogen of the Colorado potato beetle, Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae). To determine the phylogenetic status of N. leptinotarsae, the 16S SSU rRNA gene was sequenced (GenBank Accession No. MN841279) and compared phylogenetically against 21 microsporidian 16S SSU rRNA sequences using neighbour-joining and maximum-parsimony methods. The per cent identities of the N. leptinotarsae and other members of the Nosema–Vairimorpha clade ranged from 78.1 to 98.5%. Pairwise phylogenetic distances between the N. leptinotarsae and other species ranged from 0.009 to 0.320. Phylogenetic analysis shows clearly that N. leptinotarsae is a member of the Vairimorpha clade rather than the Nosema clade. The sequence divergence and morphological traits separated the N. leptinotarsae from other species in the Vairimorpha complex. As a result, a new assignment of Vairimorpha leptinotarsae comb. nov. has been implemented for N. leptinotarsae according to the phylogenetical positioning in the present study.

Keywords

ChrysomelidaeLeptinotarsa decemlineataMicrosporidiaNosema leptinotarsaepotatoVairimorpha
Type
Research Article
Information
Parasitology , Volume 147 , Issue 9 , August 2020 , pp. 1019 - 1025
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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