Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-28T05:32:50.740Z Has data issue: false hasContentIssue false
  • English
  • Français

The blocking of human antibody-dependent, eosinophil-mediated killing of Schistosoma mansoni schistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen

Published online by Cambridge University Press:  06 April 2009

D. W. Dunne ,
Q. D. Bickle ,
A. E. Butter Worth  and
B. A. Richardson
D. W. Dunne
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans
A. E. Butter Worth
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
B. A. Richardson
Affiliation:
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
Get access Rights & Permissions [Opens in a new window]

Summary

Three IgM monoclonal antibodies, M22G11P, M7B7 and M22B3G, which reacted with the surface of Schistosoma mansoni schistosomula in an indirect fluorescent antibody assay, were found to recognize a polysaccharide-containing egg antigen previously designated K3. The monoclonal antibodies and a monospecific rabbit anti-K3 serum also recognized a crossreacting antigen in a crude cercarial antigen preparation. In an eosinophil-mediated schistosomulum killing assay, all three monoclonal antibodies significantly inhibited the level of killing produced by human infection serum. An IgG3 monoclonal antibody, M22C1C, which also recognized the egg antigen K3, did not inhibit eosinophil-mediated killing. However, when lower concentrations of human serum were used in the assay, this monoclonal antibody significantly enhanced the level of killing, despite having no capacity to induce eosinophil-mediated damage in the absence of human infection serum. On the basis of these and other results we suggest the possibility that antibodies to S. mansoni egg antigens which cross-react with the surface of the early post-penetration schistosomulum may influence the effective expression of antibody-dependent, eosinophil-mediated effector mechanisms in human infections.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 269 - 280
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bickle, Q. D., Andrews, B. J. & Taylor, M. G. (1986). Schistosoma mansoni: characterisation of two protective monoclonal antibodies. Parasite Immunology 8, 95–107.CrossRefGoogle ScholarPubMed
Bickle, Q. D., Ford, M. J. & Andrews, B. J. (1983). Studies on the development of anti-schistosomular surface antibodies by the mice exposed to irradiated cercariae, adults and/or eggs of S. mansoni. Parasite Immunology 5, 499–511.CrossRefGoogle ScholarPubMed
Butterworth, A. E. (1984). Cell-mediated damage to helminths. Advances in Parasitology 23, 143–235.Google Scholar
Butterworth, A. E., Bensted-Smith, R., Capron, A., Capron, M., Dalton, P. R., Dunne, D. W., Gryzch, J. M., Kariuki, H. C., Khalife, J., Koech, D., Mugambi, M., Ouma, J. H., Arapsiongok, T. K. & Sturrock, R. F. (1987). Immunity in human schistosomiasis mansoni: prevention by blocking antibodies of the expression of immunity in young children. Parasitology 94, 281–300.Google Scholar
Butterworth, A. E., Capron, M., Cordingley, J. S., Dalton, P. R., Dunne, D. W., Kariuki, H. C., Kimani, G., Koech, D., Mugambi, M., Ouma, J., Prentice, M. A., Richardson, B. A., Arap Siongok, T. K., Sturrock, R. F. & Taylor, D. W. (1985). Immunity after treatment of human schistosomiasis mansoni. II. Identification of resistant individuals, and analysis of their immune responses. Transactions of the Royal Society of Tropical Medicine and Hygiene 79, 393–408.Google Scholar
Butterworth, A. E., Dalton, P. R., Dunne, D. W., Mugambi, M., Ouma, J. H., Richardson, B. A., Arap Siongok, T. K. & Sturrock, R. F. (1984). Immunity after treatment of human schistosomiasis mansoni. I. Study design, pretreatment observations and the results of treatment. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 108–23.Google Scholar
Butterworth, A. E., David, J. R., Franks, D., Mahmoud, A. A. F., David, P. H., Sturrock, R. F. & Houba, V. (1977). Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purified eosinophils. Journal of Experimental Medicine 145, 136–50.Google Scholar
Butterworth, A. E., Sturrock, R. F., Houba, V., Mahmoud, A. A. F., Sher, A. & Rees, P. H. (1975). Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature, London 256, 727–9.Google Scholar
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle ScholarPubMed
Dessein, A., Butterworth, A. E., Vadas, M. A. & David, J. R. (1983). Maturation in vivo of Schistosoma mansoni schistosomula after culture in vitro with granulocytes and antibody. Infection and Immunity 39, 225–32.CrossRefGoogle ScholarPubMed
Dissous, C. & Capron, A. (1981). Isolation of surface antigens from Schistosoma mansoni schistosomula. In Protides of the Biological Fluids, (ed. Peeters, H.), pp. 179–82. Oxford and New York: Pergamon Press.Google Scholar
Dissous, C., Dissous, C. & Capron, A. (1981). Isolation and characterization of surface antigens from Schistosoma mansoni schistosomula. Molecular and Biochemical Parasitology 3, 215–25.Google Scholar
Dissous, C., Grzych, J. M. & Capron, A. (1982). Schistosoma mansoni surface antigen defined by a rat monoclonal IgG2a. Journal of Immunology 129, 2232–4.Google Scholar
Dunne, D. W. & Bickle, Q. D. (1987). Identification and characterization of a polysaccharide-containing antigen from Schistosoma mansoni eggs which cross-reacts with the surface of schistosomula. Parasitology 94, 255–268.CrossRefGoogle ScholarPubMed
Dunne, D. W., Lucas, S., Bickle, Q., Pearson, S., Madgwick, L., Bain, J. & Doenhoff, M. J. (1981). Identification and partial purification of an antigen ω 1 from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 54–71.CrossRefGoogle Scholar
Grzych, J. M., Capron, M., Dissous, C. & Capron, A. (1984). Blocking antibody of rat monoclonal antibodies in experimental schistosomiasis. Journal of Immunology 133, 998–1004.Google Scholar
Harn, D. A., Mitsuyama, M. & David, J. R. (1984). Schistosoma mansoni: anti-egg monoclonal antibodies protect against cercarial challenge in vivo. Journal of Experimental Medicine 159, 1371–87.Google Scholar
Iskander, R., Das, P. K. & Aalberse, R. C. (1981). IgG4 antibodies in Egyptian patients with schistosomiasis. International Archives of Allergy and Applied Immunology 66, 200–7.Google Scholar
Khalife, J., Capron, M., Capron, A., Grzych, J.-M., Butterworth, A. E., Dunne, D. W. & Ouma, J. H. (1987). Immunity in human schistosomiasis mansoni. Regulation of protective immune mechanisms by IgM blocking antibodies. Journal of Experimental Medicine (in the Press).Google Scholar
Laemmli, U. K. (1970). cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Moore, D. V. & Sandground, J. Y. (1956). The relative egg-producing capacity of Schistosoma mansoni and Schistosoma japonicum. American Journal of Tropical Medicine and Hygiene 5, 831–40.Google Scholar
Simpson, A. J. G. & Smithers, S. R. (1985). Schistosomes: surface, egg and circulating antigens. Current Topics in Microbiology and Immunology 120, 205–39.Google ScholarPubMed
Tarrab-Hazdai, R., Levi-Schaffer, F., Brenner, V., Horowitz, S., Eshhar, Z. & Aron, R. (1985). Protective monoclonal antibody against Schistosoma mansoni: antigen isolation, characterization, and suitability for active immunization. Journal of Immunology 135, 2772–9.Google Scholar
Vadas, M. A., David, J. R., Butterworth, A. E., Pisani, N. T. & Arap Siongok, T. K. (1979). A new method for purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. Journal of Immunology 122, 1228–36.Google Scholar
Warren, K. S. (1980). Schistosome eggs as antigenic depots: production, secretion, destruction. In Cancer Immunology and Parasite Immunology INSERM 97, 449–60.Google Scholar
Warren, K. S., Mahmoud, A. A. F., Cumminos, P., Murphy, D. J. & Houser, H. B. (1974). Schistosomiasis mansoni in Yemeni in California. Duration of infection, presence of disease, therapeutic management. American Journal of Tropical Medicine and Hygiene 23, 902–9.Google Scholar
Zodda, D. M. & Phillips, S. M. (1982). Monoclonal antibody-mediated protection against Schistosoma mansoni infection in mice. Journal of Immunology 129, 2326–8.Google Scholar