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Characterization of actin genes in Bonamia ostreae and their application to phylogeny of the Haplosporidia

Published online by Cambridge University Press:  03 August 2007

I. LÓPEZ-FLORES ,
V. N. SUÁREZ-SANTIAGO ,
D. LONGET ,
D. SAULNIER ,
B. CHOLLET  and
I. ARZUL
I. LÓPEZ-FLORES*
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
V. N. SUÁREZ-SANTIAGO
Affiliation:
Department of Botany, Faculty of Sciences, University of Granada, Avenida Severo Ochoa s/n, 18071 Granada, Spain
D. LONGET
Affiliation:
Department of Zoology and Animal Biology, University of Geneva, Sciences III, 30 Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
D. SAULNIER
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
B. CHOLLET
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
I. ARZUL
Affiliation:
Laboratoire de Génétique et Pathologie. Station La Tremblade, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France
*
*Corresponding author: Present address: Laboratoire de Génétique et Pathologie, IFREMER. Avenue Mus de Loup, 17390 La Tremblade, France. Tel: +33 546762647. Fax: +33 546762611. E-mail: ilopez@ugr.es
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Summary

Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and demonstrated that the paralogous actin genes found in Bonamia resulted from a duplication of the original actin gene after the Bonamia origin.

Keywords

Bonamia ostreaeOstrea edulis parasiteactin genemolecular phylogeny
Type
Research Article
Information
Parasitology , Volume 134 , Issue 14 , December 2007 , pp. 1941 - 1948
Copyright
Copyright © Cambridge University Press 2007

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References

REFERENCES

Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein data base search programs. Nucleic Acids Research 25, 3389–3402. doi: 10.1093/nar/25.17.3389.CrossRefGoogle Scholar
Burreson, E. M., Stokes, N. A., Carnegie, R. B. and Bishop, M. J. (2004). Bonamia sp. (Haplosporidia) found in nonnative oysters Crassostrea ariakensis in Bogue Sound, North Carolina. Journal of Aquatic Animal Health 16, 1–9. doi: 10.1577/H03-008.1.CrossRefGoogle Scholar
Campalans, M., Rojas, P. and González, M. (2000). Haemocytic parasitosis in the farmed oyster Tiostrea chilensis. Bulletin of the European Association of Fish Pathologists 20, 31–33.Google Scholar
Carnegie, R. B., Barber, B. J., Culloty, S. C., Figueras, A. J. and Distel, D. L. (2000). Development of a PCR assay for detection of the oyster pathogen Bonamia ostreae and support for its inclusion in the Haplosporidia. Diseases of Aquatic Organisms 42, 199–206.CrossRefGoogle ScholarPubMed
Carnegie, R. B., Burreson, E. M., Hine, M., Stokes, N. A., Audemard, C., Bishop, M. J. and Peterson, C. H. (2006). Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores. The Journal of Eukaryotic Microbiology 53, 232–245. doi: 10.1111/j.1550-7408.2006.00100.X.CrossRefGoogle Scholar
Carnegie, R. B. and Cochennec-Laureau, N. (2004). Microcell parasites of oysters: Recent insights and future trends. Aquatic Living Resources 17, 519–528. doi: 10.1051/alr:2004055.CrossRefGoogle Scholar
Cochennec, N., Le Roux, F., Berthe, F. and Gerard, A. (2000). Detection of Bonamia ostreae based on small subunit ribosomal probe. Journal of Invertebrate Pathology 76, 26–32. doi: 10.1006/jipa.2000.4939.CrossRefGoogle ScholarPubMed
Corbeil, S., Arzul, I., Robert, M., Berthe, F. C. J., Besnard-Cochennec, N. and Crane, M. ST. J. (2006). Molecular characterisation of an Australian isolate of Bonamia exitiosa. Diseases of Aquatic Organisms 71, 81–85.CrossRefGoogle ScholarPubMed
Farley, C. A., Wolf, P. H. and Elston, R. (1988). A long-term study of “microcell” disease in oysters with a description of a new genus, Mikrocytos (g. n.), and two new species, Mikrocytos mackini (sp. n.) and Mikrocytos roughleyi (sp. n.). Fishery Bulletin 86, 581–593.Google Scholar
Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.CrossRefGoogle ScholarPubMed
Flakowski, J., Bolivar, I., Fahrni, J. and Pawlowski, J. (2006). Tempo and mode of spliceosomal intron evolution in actin of Foraminifera. Journal of Molecular Evolution 63, 30–41. doi: 10.1007/s00239-005-0061-z.CrossRefGoogle ScholarPubMed
Hightower, R. C. and Meagher, R. B. (1986). The molecular evolution of actin. Genetics 114, 315–332.CrossRefGoogle ScholarPubMed
Hine, P. M., Cochennec-Laureau, N. and Berthe, F. C. (2001). Bonamia exitiosa n. sp. (Haplosporidia) infecting flat oysters, Ostrea chilensis, in New Zealand. Diseases of Aquatic Organisms 47, 63–72.CrossRefGoogle Scholar
Huber, M., Garfinkel, L., Gitler, C., Mirelman, D., Revel, M. and Rozenblatt, S. (1987). Entamoeba histolytica: cloning and characterization of actin cDNA. Molecular and Biochemical Parasitology 24, 227–235. doi: 10.1016/0166-6851(87)90154-X.CrossRefGoogle ScholarPubMed
Kroeck, M. A. and Montes, J. (2005). Occurrence of the haemocyte parasite Bonamia sp. in flat oysters Ostrea puelchana farmed in San Antonio Bay (Argentina). Diseases of Aquatic Organisms 63, 231–235.CrossRefGoogle Scholar
Longet, D., Burki, F., Flakowski, J., Berney, C., Polet, S., Fahrni, J. and Pawlowski, J. (2004). Multigene evidence for close evolutionary relations between Gromia and Foraminifera. Acta Protozoologica 43, 303–311.Google Scholar
Marty, G. D., Bower, S. M., Clarke, K. R., Meyer, G., Lowe, G., Osborn, A. L., Chow, E. P., Hannah, H., Byrne, S., Sojonky, K. and Robinson, J. H. (2006). Histopatology and real time PCR assay for detection of Bonamia ostrea in Ostrea edulis cultured in western Canada. Aquaculture 261, 33–42. doi: 10.1016/j.aquaculture.2006.07.024.CrossRefGoogle Scholar
Pawlowski, J., Bolivar, I., Fahrni, J., de Vargas, C. and Bowser, S. S. (1999). Naked foraminiferans revealed. Nature, London 399, 27. doi: 10.1038/19881.CrossRefGoogle Scholar
Pichot, Y., Comps, M., Tigé, G., Grizel, H. and Rabouin, M. A. (1980). Recherche sur Bonamia ostreae gen. n., sp. n., parasite nouveau de l'huître plate Ostrea edulis L. Revue des Travaux de l'Institut des Pêches Maritimes 43, 131–140.Google Scholar
Reece, K., Siddall, M. E., Burreson, E. M. and Graves, J. E. (1997). Phylogenetic analysis of Perkinsus based on actin gene sequences. Journal of Parasitology 83, 417–423.CrossRefGoogle ScholarPubMed
Reece, K., Siddall, M. E., Stokes, N. A. and Burreson, E. M. (2004). Molecular phylogeny of the Haplosporidia based on two independent gene sequences. Journal of Parasitology 90, 1111–1122. doi: 10.1645/GE-102R.CrossRefGoogle ScholarPubMed
Sheterline, P. and Sparrow, J. C. (1994). Actin. Protein Profile 1, 1–121.Google ScholarPubMed
Swofford, D. L. (2003) PAUP*. Phylogenetic Analysis using Parsimony (*and other Methods), Version 4. Sinauer Associates, Sunderland, Massachusetts, USA.Google Scholar
Wesseling, J. G., Smits, M. A. and Schoenmakers, J. G. G. (1988). Extremely diverged actin proteins in Plasmodium falciparum. Molecular and Biochemical Parasitology 30, 143–154. doi: 10.1016/0166–6851(88)90107-7.CrossRefGoogle ScholarPubMed
Winnepenninckx, B., Backeljau, T. and de Wachter, R. (1993). Extraction of high molecular weight DNA from molluscs. Trends in Genetics 9, 407. doi: 10.1016/0168-9525(93)90102-N.Google ScholarPubMed
Zhou, J., Huang, B., Suzuki, H., Fujisaki, K., Igarashi, I. and Xuan, X. (2006). Isolation and identification of an actin gene from Babesia gibsoni. Journal of Parasitology 92, 208–210. doi: 10.1645/GE-577R2.1.CrossRefGoogle ScholarPubMed