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Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

Published online by Cambridge University Press:  01 December 1997

J. ARAYA ,
M. I. CANO ,
H. B. M. GOMES ,
E. M. NOVAK ,
J. M. REQUENA ,
C. ALONSO ,
M. J. LEVIN ,
P. GUEVARA ,
J. L. RAMIREZ  and
J. FRANCO DA SILVEIRA
J. ARAYA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil Unidad de Parasitologia, Universidad de Antofagasta, Av. Coloso s/n, Casilla 170, Antofagasta, Chile
M. I. CANO
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
H. B. M. GOMES
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
E. M. NOVAK
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
J. M. REQUENA
Affiliation:
Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Canto Blanco 28049, Madrid, Spain
C. ALONSO
Affiliation:
Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Canto Blanco 28049, Madrid, Spain
M. J. LEVIN
Affiliation:
Ingebi, Obligado 2490, 1428 Buenos Aires, Argentina
P. GUEVARA
Affiliation:
Centro De Biologia Celular, UCV, Apdo 47525, Caracas, Venezuela
J. L. RAMIREZ
Affiliation:
Centro De Biologia Celular, UCV, Apdo 47525, Caracas, Venezuela
J. FRANCO DA SILVEIRA
Affiliation:
Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu, 862-CEP 04023-062, SP, Brazil
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Abstract

We report the molecular characterization of a middle repetitive DNA sequence, named C6, isolated from the Trypanosoma cruzi genome. C6 appears to be a composite repeated element since 3 subregions may be defined within it on the basis of sequence similarities with other T. cruzi genomic sequences. Sequences homologous to C6 are interspersed in the genome and can be mapped out on most chromosomal bands of different T. cruzi strains. The copy number of the C6 element is about 1000 per haploid genome. Given the species specificity and different genomic distribution of C6 homologous sequences among the T. cruzi strains the C6 element could be a useful probe for diagnosis and typing of parasites. C6 is a polymorphic marker with potential as a tool for physical mapping of the T. cruzi genome.

Keywords

Trypanosoma cruzi repetitive DNAsequence analysisgenomic distributionpolymorphic markerdiagnostic tool
Type
Research Article
Information
Parasitology , Volume 115 , Issue 6 , December 1997 , pp. 563 - 570
Copyright
1997 Cambridge University Press

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