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Characterization of SSU and LSU rRNA genes of three Trypanosoma (Herpetosoma) grosi isolates maintained in Mongolian jirds

Published online by Cambridge University Press:  07 October 2004

H. SATO ,
A. OSANAI ,
H. KAMIYA ,
Y. OBARA ,
W. JIANG ,
Q. ZHEN ,
J. CHAI ,
Y. UNE  and
M. ITO
H. SATO
Affiliation:
Department of Parasitology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan
A. OSANAI
Affiliation:
Department of Parasitology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan
H. KAMIYA
Affiliation:
Department of Parasitology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan
Y. OBARA
Affiliation:
Laboratory of Cytogenetics, Department of Biofunctional Science, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan
W. JIANG
Affiliation:
Xinjiang Center of Diseases Prevention and Control, Urumqi, Xinjiang 83002, China
Q. ZHEN
Affiliation:
Xinjiang Center of Diseases Prevention and Control, Urumqi, Xinjiang 83002, China
J. CHAI
Affiliation:
Xinjiang Center of Diseases Prevention and Control, Urumqi, Xinjiang 83002, China
Y. UNE
Affiliation:
Laboratory of Veterinary Pathology, School of Verinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara 229-8501, Japan
M. ITO
Affiliation:
Laboratory of Immunology, Central Institute for Experimental Animals, Kawasaki 216-0001, Japan
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Abstract

Trypanosoma (Herpetosoma) grosi, which naturally parasitizes Apodemus spp., can experimentally infect Mongolian jirds (Meriones unguiculatus). Three isolates from A. agrarius, A. peninsulae, and A. speciosus (named SESUJI, HANTO, and AKHA isolates, respectively) of different geographical origin (AKHA from Japan, and the others from Vladivostok), exhibited different durations of parasitaemia in laboratory jirds (2 weeks for HANTO, and 3 weeks for the others). To assess the genetic background of these T. grosi isolates, their small (SSU) and large subunit (LSU) ribosomal RNA genes (rDNA) were sequenced along with those of 2 other Herpetosoma species from squirrels. The SSU rDNA sequences of these 3 species along with available sequences of 3 other Herpetosoma trypanosomes (T. lewisi, T. musculi and T. microti) seemed to reflect well the phylogenetic relationship of their hosts. Three isolates of T. grosi exhibited base changes at 2–6 positions of 2019-base 18S rDNA, at 5–29 positions of 1817/1818-base 28Sα rDNA, or 1–5 positions of 1557–1559-base 28Sβ rDNA, and none was separated from the other 2 isolates by rDNA nucleotide sequences. Since base changes of Herpetosoma trypanosomes at the level of inter- and intra-species might occur frequently in specified rDNA regions, the molecular analysis on these regions of rodent trypanosomes could help species/strain differentiation and systematic revision of Herpetosoma trypanosome species, which must be more abundant than presently known.

Keywords

Herpetosoma trypanosomesTrypanosoma grosiSSU rDNALSU rDNAMeriones unguiculatusApodemus spp
Type
Research Article
Information
Parasitology , Volume 130 , Issue 2 , February 2005 , pp. 157 - 167
Copyright
© 2005 Cambridge University Press

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