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Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum

Published online by Cambridge University Press:  25 February 2008

L. MOERTEL ,
G. N. GOBERT  and
D. P. McMANUS
L. MOERTEL
Affiliation:
Queensland Institute of Medical Research, and Australian Centre for International Health and Nutrition, Herston, QLD 4029, Australia
G. N. GOBERT*
Affiliation:
Queensland Institute of Medical Research, and Australian Centre for International Health and Nutrition, Herston, QLD 4029, Australia
D. P. McMANUS
Affiliation:
Queensland Institute of Medical Research, and Australian Centre for International Health and Nutrition, Herston, QLD 4029, Australia
*
*Corresponding author: Tel: +61 7 33620406. Fax: +61 7 33620104. E-mail: geoffG@qimr.edu.au
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Summary

Schistosomes are complex parasitic helminths with discrete life-cycle stages, adapted for survival in their mammalian and snail hosts and the external aquatic environment. Recently, we described the fabrication and use of a microarray to investigate gender-specific transcription in Schistosoma japonicum. To address transcriptional differences, 8 gender-associated gene transcripts identified previously by the microarray analysis were selected for further study. First, differential transcription patterns were investigated in 4 developmental stages using real-time PCR. Subsequently, we undertook functional analysis of a subset of 4 transcripts encoding metabolic enzymes, so as to correlate gender-associated transcript levels with enzyme activity in protein extracts from adult worms. The 8 characterized molecules serve as a basis for further investigation of differential gene expression during the schistosome life-cycle and for studying the sexual dimorphism of adult worms. Continual refinement and annotation of the microarray used in the current study should support future work on these aspects.

Keywords

SchistosomaSchistosoma japonicummicroarrayreal-time PCRgene expression
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 5 , May 2008 , pp. 575 - 583
Copyright
Copyright © Cambridge University Press 2008

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