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Comparison of cysteine peptidase activities in Trichobilharzia regenti and Schistosoma mansoni cercariae

Published online by Cambridge University Press:  22 May 2007

M. KAŠNÝ ,
L. MIKEŠ ,
J. P. DALTON ,
A. P. MOUNTFORD  and
P. HORÁK
M. KAŠNÝ*
Affiliation:
Charles University in Prague, Faculty of Science, Department of Parasitology, Viničná 7, 12844 Prague 2, Czech Republic
L. MIKEŠ
Affiliation:
Charles University in Prague, Faculty of Science, Department of Parasitology, Viničná 7, 12844 Prague 2, Czech Republic
J. P. DALTON
Affiliation:
Institute for the Biotechnology of Infectious Diseases, University of Technology Sydney, PO Box 123, Broadway, N.S.W. 2007 Sydney, Australia
A. P. MOUNTFORD
Affiliation:
Department of Biology (Area 5), University of York, P.O. Box 373, York YO10 5YW, UK
P. HORÁK
Affiliation:
Charles University in Prague, Faculty of Science, Department of Parasitology, Viničná 7, 12844 Prague 2, Czech Republic
*
*Corresponding author: Charles University in Prague, Faculty of Science, Department of Parasitology, Viničná 7, 12844 Prague 2, Czech Republic. Tel: +420 221 951 816. Fax: +420 224 919 704. E-mail: kasa@post.cz
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Summary

Cercariae of the bird schistosome Trichobilharzia regenti and of the human schistosome Schistosoma mansoni employ proteases to invade the skin of their definitive hosts. To investigate whether a similar proteolytic mechanism is used by both species, cercarial extracts of T. regenti and S. mansoni were biochemically characterized, with the primary focus on cysteine peptidases. A similar pattern of cysteine peptidase activities was detected by zymography of cercarial extracts and their chromatographic fractions from T. regenti and S. mansoni. The greatest peptidase activity was recorded in both species against the fluorogenic peptide substrate Z-Phe-Arg-AMC, commonly used to detect cathepsins B and L, and was markedly inhibited (>96%) by Z-Phe-Ala-CHN2 at pH 4·5. Cysteine peptidases of 33 kDa and 33–34 kDa were identified in extracts of T. regenti and S. mansoni cercariae employing a biotinylated Clan CA cysteine peptidase-specific inhibitor (DCG-04). Finally, cercarial extracts from both T. regenti and S. mansoni were able to degrade native substrates present in skin (collagen II and IV, keratin) at physiological pH suggesting that cysteine peptidases are important in the pentration of host skin.

Keywords

TrichobilharziaSchistosomacercariacysteine peptidaseproteasepenetrationDCG-04
Type
Research Article
Information
Parasitology , Volume 134 , Issue 11 , October 2007 , pp. 1599 - 1609
Copyright
Copyright © Cambridge University Press 2007

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