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Comparison of isolates and species of Toxocara and Toxascaris by biosynthetic labelling of somatic and ES proteins from infective larvae

Published online by Cambridge University Press:  06 April 2009

A. P. Page ,
D. T. Richards ,
J. W. Lewis ,
H. M. Omar  and
R. M. Maizels
A. P. Page
Affiliation:
Wellcome Research Centre for Parasitic Infections, Department of Biology, Imperial College of Science Technology and Medicine, London
D. T. Richards
Affiliation:
Department of Biology, Royal Holloway and Bedford New College, Egham, Surrey
J. W. Lewis
Affiliation:
Department of Biology, Royal Holloway and Bedford New College, Egham, Surrey
H. M. Omar
Affiliation:
Department of Parasitology, College of Veterinary Medicine, Giza, Cairo, Egypt
R. M. Maizels
Affiliation:
Wellcome Research Centre for Parasitic Infections, Department of Biology, Imperial College of Science Technology and Medicine, London
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Extract

Infective-stage larvae of three different isolates of Toxocara canis were intrinsically ([35S]methionine) labelled in culture, to determine the presence of similarities or differences in the somatic and ES antigens expressed between larvae derived from different hosts and different geographical regions. Two other closely related ascaridids, Toxascaris leonina which infects cats and dogs, and Toxocara vitulorum (Neoascaris vitulorum) which infects cattle, were also compared to T. canis larvae by this method. Overall comparisons were made by 1- and 2-dimensional electrophoresis, while immunological cross-reactivities between the different species were analysed by radio-immunoprecipitation. Our results show that extensive physicochemical characteristics are shared between T. canis isolates, both from different hosts and different geographical locations. A substantial overlap was revealed when T. canis and T. vitulorum antigens were compared, whereas Toxascaris was found to produce a distinct antigen profile: this result was independent of whether methionine- or Iodogen-labelled products were being considered. Antigen recognition by polyclonal antibodies raised to all three species and to the cat ascaridid Toxocara cati, revealed considerable cross-reactivities. The cross-reactions were especially prominent between the Toxocara species, a fact further substantiated when reactivity of T. canis ES-specific monoclonal antibodies were tested against T. leonina and T. vitulorum antigens. The ES antigens of T. leonina were not recognized by the T. canis monoclonals, whereas the majority of these antibodies precipitated antigens of T. vitulorum. One which did not react with T. vitulorum was monoclonal antibody Tcn 2, indicating its species-specific reactivity and therefore its potential for the specific diagnosis of human toxocariasis.

Keywords

ToxocaraToxascarisisolatesspeciesantigensdiagnosis
Type
Research Article
Information
Parasitology , Volume 103 , Issue 3 , December 1991 , pp. 451 - 464
Copyright
Copyright © Cambridge University Press 1991

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