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Correlation of structural development and differential expression of invasion-related molecules in schizonts of Plasmodium falciparum

Published online by Cambridge University Press:  23 August 2004

G. MARGOS ,
L. H. BANNISTER ,
A. R. DLUZEWSKI ,
J. HOPKINS ,
I. T. WILLIAMS  and
G. H. MITCHELL
G. MARGOS
Affiliation:
Department of Immunobiology, Guy's, Kings, and St Thomas' Schools of Medicine, Guy's Hospital, London SE1 9RT, UK
L. H. BANNISTER
Affiliation:
Department of Anatomy, Cell and Human Biology, GKT, School of Biomedical Sciences, London SE1 1UL, UK
A. R. DLUZEWSKI
Affiliation:
Department of Immunobiology, Guy's, Kings, and St Thomas' Schools of Medicine, Guy's Hospital, London SE1 9RT, UK
J. HOPKINS
Affiliation:
Department of Anatomy, Cell and Human Biology, GKT, School of Biomedical Sciences, London SE1 1UL, UK
I. T. WILLIAMS
Affiliation:
Department of Immunobiology, Guy's, Kings, and St Thomas' Schools of Medicine, Guy's Hospital, London SE1 9RT, UK
G. H. MITCHELL
Affiliation:
Department of Immunobiology, Guy's, Kings, and St Thomas' Schools of Medicine, Guy's Hospital, London SE1 9RT, UK
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Abstract

During asexual development Plasmodium schizonts undergo a series of complex biochemical and structural changes. Using tightly synchronized cultures of 2 P. falciparum lines (clone C10 and strain ITO4) for light microscopy and fluorescence imaging we monitored the timing and sequence of expression of proteins associated with invasion-related organelles. Antibodies to rhoptry, micronemal and dense granule proteins (Rhoptry Associated Protein 1, Apical Membrane Antigen 1, Erythrocyte Binding Antigen 175, Ring-infected Erythrocyte Surface Antigen) and to pellicle-associated proteins (Merozoite Surface Protein 1, PfMyosin-A) were used. Clone C10 developed faster than ITO4; this difference was also found in the timing of protein expression seen by immunofluorescence. Light microscopic data were combined with transmission electron microscopic analysis using serial sectioning of ITO4 schizonts to determine nuclear number and organellar development. Thus a timetable of schizont structural maturation was established. Generally, the timing of organelle-specific antigen expression correlates well with the ultrastructural data. Rhoptries are formed mainly between second and fourth nuclear divisions, micronemes between the end of the fourth nuclear division and merozoite separation from the residual body, while dense granules are generated mainly after the micronemes. PfAMA-1 appears in micronemes before EBA-175, suggesting micronemal heterogeneity.

Keywords

Plasmodium falciparumschizontmerozoite developmentimmunolocalizationultrastructuretime-courseorganelles
Type
Research Article
Information
Parasitology , Volume 129 , Issue 3 , September 2004 , pp. 273 - 287
Copyright
© 2004 Cambridge University Press

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