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Cultivation of Trypanosoma cruzi in irradiated muscle cells: improved synchronization and enhanced trypomastigote production

Published online by Cambridge University Press:  06 April 2009

D. M. Schmatz  and
P. K. Murray
D. M. Schmatz
Affiliation:
Department of Immunoparasitology, Merck Institute for Therapeutic Research, Rahway, New Jersey 07065
P. K. Murray
Affiliation:
Department of Immunoparasitology, Merck Institute for Therapeutic Research, Rahway, New Jersey 07065
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Summary

Since the in vitro production of trypomastigote stages of Trypanosoma cruzi in cell culture is frequently limited by (1) host cell overgrowth and (2) by the unequal redistribution of parasites after cell division resulting in asynchronous release of trypomastigotes, a culture system was devised in which host cell mitosis was inhibited by irradiation prior to parasite infection. L-6 rat myoblast cells when exposed to 3000 rad. of gamma radiation lost their ability to divide but remained susceptible to infection with, and capable of supporting the intracellular growth of, T. cruzi. Using this approach it proved possible to have virtually 100% of cells infected and achieve much better synchronization of trypomastigote release than with conventional culture systems. Additionally, the total number of parasites provided approached 1 × 109 trypomastigotes/ 150 cm2 flask, a significant increase over other culture systems. Preliminary studies with Plasmodium fallax and Eimeria tenella indicate that irradiated host cells may be utilized to advantage for the cultivation of other intracellular protozoa.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 1 , August 1982 , pp. 115 - 125
Copyright
Copyright © Cambridge University Press 1982

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