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Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites

Published online by Cambridge University Press:  22 February 2005

A. HUDSON  and
J. T. ELLIS
A. HUDSON
Affiliation:
Department of Cell and Molecular Biology, University of Technology, Sydney, St Leonard's Campus, Westbourne Street, Gore Hill, NSW 2065, Australia
J. T. ELLIS
Affiliation:
Department of Cell and Molecular Biology, University of Technology, Sydney, St Leonard's Campus, Westbourne Street, Gore Hill, NSW 2065, Australia
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Abstract

Mycoplasmas are common contaminants of eukaryotic cells grown in tissue culture. A commercially available Mycoplasma Removal Agent (MRA) was therefore assessed for its effect on tachyzoites of Neospora caninum, in order to determine its suitability for further use in parasite cell cultures. Analyses of tachyzoite and excreted-secreted proteins and antigens by SDS-PAGE and Western blotting show that MRA treatment results in the rapid selection of a mutant population that differs from the control and parental lines in its protein and antigen content. The treatment of N. caninum cultures with MRA is therefore not recommended for the eradication of Mycoplasma.

Keywords

Neospora caninumMycoplasmaantibioticquinoloneculture
Type
Research Article
Information
Parasitology , Volume 130 , Issue 6 , June 2005 , pp. 607 - 610
Copyright
© 2005 Cambridge University Press

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