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Demasculinization of male guppies increases resistance to a common and harmful ectoparasite

Published online by Cambridge University Press:  24 September 2015

FELIPE DARGENT ,
ADAM R. REDDON ,
WILLIAM T. SWANEY ,
GREGOR F. FUSSMANN ,
SIMON M. READER ,
MARILYN E. SCOTT  and
MARK R. FORBES
FELIPE DARGENT*
Affiliation:
Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, ON, Canada
ADAM R. REDDON
Affiliation:
Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada
WILLIAM T. SWANEY
Affiliation:
Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, UK
GREGOR F. FUSSMANN
Affiliation:
Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada
SIMON M. READER
Affiliation:
Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada
MARILYN E. SCOTT
Affiliation:
Institute of Parasitology and Centre for Host-Parasite Interactions, McGill University, 21111 Lakeshore Road, Ste-Anne de Bellevue, H9X 3V9, QC, Canada
MARK R. FORBES
Affiliation:
Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, ON, Canada
*
*Corresponding author. Department of Biology, McGill University, 1205 Dr. Penfield Av., Montreal, H3A 1B1, QC, Canada, Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, K1S 5B6, ON, Canada. E-mail: felipe.dargent@mail.mcgill.ca
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Summary

Parasites are detrimental to host fitness and therefore should strongly select for host defence mechanisms. Yet, hosts vary considerably in their observed parasite loads. One notable source of inter-individual variation in parasitism is host sex. Such variation could be caused by the immunomodulatory effects of gonadal steroids. Here we assess the influence of gonadal steroids on the ability of guppies (Poecilia reticulata) to defend themselves against a common and deleterious parasite (Gyrodactylus turnbulli). Adult male guppies underwent 31 days of artificial demasculinization with the androgen receptor-antagonist flutamide, or feminization with a combination of flutamide and the synthetic oestrogen 17 β-estradiol, and their parasite loads were compared over time to untreated males and females. Both demasculinized and feminized male guppies had lower G. turnbulli loads than the untreated males and females, but this effect appeared to be mainly the result of demasculinization, with feminization having no additional measurable effect. Furthermore, demasculinized males, feminized males and untreated females all suffered lower Gyrodactylus-induced mortality than untreated males. Together, these results suggest that androgens reduce the ability of guppies to control parasite loads, and modulate resistance to and survival from infection. We discuss the relevance of these findings for understanding constraints on the evolution of resistance in guppies and other vertebrates.

Keywords

gonadal steroidsfeminizationsex-biased parasitismtestosteronefishGyrodactylusPoecilia reticulata
Type
Research Article
Information
Parasitology , Volume 142 , Issue 13 , November 2015 , pp. 1647 - 1655
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Copyright
Copyright © Cambridge University Press 2015 

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