Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T21:57:19.721Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of coproantibodies and faecal immune complexes in human trichinellosis

Published online by Cambridge University Press:  11 December 2006

G. G. NUÑEZ ,
S. N. COSTANTINO  and
S. M. VENTURIELLO
G. G. NUÑEZ
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
S. N. COSTANTINO
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
S. M. VENTURIELLO*
Affiliation:
Chair of Immunology, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina-“Prof. Dr. R. A. Margni” Humoral Immunity Studies Institute, CONICET. Junín 956, (1113) Buenos Aires, Argentina
*
*Corresponding author: Cátedra de Inmunología, Departamento de Microbiología, Inmunología y Biotecnología, Facultad de Farmacia y Bioquímica, Junín 956, (1113) Buenos Aires, Argentina. Tel: +54 11 4964 8259. Fax: +5411 4964 0024. E-mail: sventuri@huemul.ffyb.uba.ar
Get access Rights & Permissions [Opens in a new window]

Summary

Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis.

Keywords

human trichinellosiscoproantibodiescoproantigensfaecal immune complexesimmunoglobulin isotypes
Type
Research Article
Information
Parasitology , Volume 134 , Issue 5 , May 2006 , pp. 723 - 727
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abdel Baki, M. H., Younis, T. A., Habib, K. S., Ramadan, N. I., Metwally, M. D., Ismail, K. A. and Saber, M. M. (2004). Molecular identification of coproantigens of Cryptosporidium parvum compared to conventional staining techniques. Journal of the Egyptian Society of Parasitology 34, 967–978.Google ScholarPubMed
Ahmad, A., Wang, C. H. and Bell, R. G. (1991). A role for IgE in intestinal immunity. Expression of rapid expulsion of Trichinella spiralis in rats transfused with IgE and thoracic duct lymphocytes. Journal of Immunology 146, 3563–3570.CrossRefGoogle ScholarPubMed
Allan, J. C., Wilkins, P. P., Tsang, V. C. and Craig, P. S. (2003). Immunodiagnostic tools for taeniasis. Acta Tropica 87, 87–93.CrossRefGoogle ScholarPubMed
Appleton, J. A., Bell, R. G., Homan, W. and van Knapen, F. (1991). Consensus on Trichinella spiralis antigens and antibodies. Parasitology Today 7, 190–192.CrossRefGoogle Scholar
Bell, R. G. (1998). The generation and expression of immunity to Trichinella spiralis in laboratory rodents. Advances in Parasitology 41, 149–217.CrossRefGoogle ScholarPubMed
Costantino, S. N., Malmassari, S. L., Dalla Fontana, M. L., Diamante, M. A. and Venturiello, S. M. (2001). Diagnosis of human trichinellosis: pitfalls in the use of a unique immunoserological technique. Parasite 8, S144–S146.CrossRefGoogle ScholarPubMed
Dupouy-Camet, J., Kociecka, W., Bruschi, F., Bolas-Fernandez, F. and Pozio, E. (2002). Opinion on the diagnosis and treatment of human trichinellosis. Expert Opinion in Pharmacotherapy 3, 1117–1130.Google ScholarPubMed
Espino, A. M. and Finlay, C. M. (1994). Sandwich enzyme-linked immunosorbent assay for detection of excretory-secretory antigens in humans with fasciolasis. Journal of Clinical Microbiology 32, 190–193.CrossRefGoogle Scholar
Espino, A. M., Diaz, A., Perez, A. and Finlay, C. M. (1998). Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak. Journal of Clinical Microbiology 36, 2723–2726.CrossRefGoogle ScholarPubMed
Gamble, H. R., Anderson, W. R., Graham, C. E. and Murrell, K. D. (1983). Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory-secretory antigen. Veterinary Parasitology 13, 349–361.CrossRefGoogle ScholarPubMed
Jamornthanyawat, N. (2002). The diagnosis of human opisthorchiasis. Southeast Asian Journal of Tropical Medicine and Public Health 33, 86–91.Google ScholarPubMed
Murrell, K. D. and Bruschi, F. (1994). Clinical trichinellosis. In Progress in Clinical Parasitology (ed. Tsieh Sun, M. D.), pp. 117–150. CRC Press, New York.Google Scholar
Negrão-Corrêa, D., Adams, L. S. and Bell, R. G. (1996). Intestinal transport and catabolism of IgE. A major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection in rats. Journal of Immunology 157, 4037–4044.CrossRefGoogle Scholar
Negrão-Corrêa, D., Adams, L. S. and Bell, R. G. (1999). Variability of the intestinal immunoglobulin E response of rats to infection with Trichinella spiralis, Heligmosomoides polygyrus or Nippostrongylus brasiliensis. Parasite Immunology 21, 287–297.CrossRefGoogle ScholarPubMed
Nuñez, G. G., Costantino, S. N. and Venturiello, S. M. (2003). Immunoparasitological parameters of the intestinal phase of trichinellosis in rats. Parasitology 126, 321–325.CrossRefGoogle ScholarPubMed
Nuñez, G. G., Malmassari, S. L., Costantino, S. N. and Venturiello, S. M. (2000). Immunoelectrotransfer blot assay in acute and chronic human trichinellosis. Journal of Parasitology 85, 1121–1124.CrossRefGoogle Scholar
Pawlowski, Z. S. (1983). Clinical aspects in man. In Trichinella and Trichinosis (ed. Campbell, W. C.), pp. 367–401. Plenum Press, New York.CrossRefGoogle Scholar
Soulé, C. and Dupouy-Camet, J. (1991). Aspects cliniques et traitement de la trichinellose chez l'homme. In La Trichinellose: une Zoonose en Évolution (ed. Soulé, C. and Dupouy-Camet, J.), pp. 145–169. Office International des Epizooties, Paris.Google Scholar