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Detection of host DNA sequences including the H-2 locus of the major histocompatibility complex in schistosomes

Published online by Cambridge University Press:  06 April 2009

Y. Iwamura ,
H. Yonekawa  and
Y. Irie
Y. Iwamura
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki-ken 305, Japan
H. Yonekawa
Affiliation:
Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Sciences, Bunkyo-ku, Tokyo 113, Japan
Y. Irie
Affiliation:
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba-shi, Ibaraki-ken 305, Japan
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Summary

The mouse type 2 Alu (B2) sequence was detected in both DNAs of Schistosoma mansoni and S. japonicum except for the cercarial stage by the polymerase chain reaction (PCR). Using several kinds of mouse STMS (sequence tagged microsatellite site) primer sets, PCR products related to the host were found in the DNAs of S. mansoni as well as of S. japonicum. Products could be detected only in the DNA of S. japonicum using certain STMS primer sets. The fact that no products could be amplified from the DNAs of both parasites when other kinds of STMS primer sets were used suggests unequal incorporation of the host DNA into the schistosomes. Furthermore, the sequence of the N-terminal domain of H-2, the mouse major histocompatibility complex (MHC), was detected in the DNAs from S. mansoni miracidium, male adult and S. japonicum adults, whereas the sequence of the C2 domain of H-2 was found only in the DNAs of S. japonicum adults. This evidence that host DNA sequences, including the class I MHC, exist heterogeneously in the DNAs of schistosomes might provide an important insight for further understanding of host-parasite immune interactions.

Keywords

Schistosoma mansoniSchistosoma japonicummouse microsatellitespolymerase chain reactionmajor histocompatibility complex
Type
Research Article
Information
Parasitology , Volume 110 , Issue 2 , February 1995 , pp. 163 - 170
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Aitmen, T. J., Hearne, C. M., McAleer, M. A. & Todd, J. A. (1991). Mononucleotide repeats are an abundant source of length variants in mouse genomic DNA. Mammalian Genome 1, 206–10.CrossRefGoogle Scholar
Coligan, J. E., Kindt, T. J., Nairn, R., Nathenson, S. G., Sachs, D. H. & Hansen, T. H. (1980). Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens. Proceedings of the National Academy of Sciences, USA 77, 1134–8.CrossRefGoogle ScholarPubMed
Damian, R. T. (1987). Molecular mimicry revisited. Parasitology Today 3, 263–6.CrossRefGoogle ScholarPubMed
Dietrich, W., Katz, H., Lincoln, S. E., Shin, H. S., Friedman, J., Dracopoli, N. C. & Lander, E. S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–47.CrossRefGoogle ScholarPubMed
Dresden, M. H. & Payne, D. c. (1981). A sieving method for the collection of schistosome eggs from mouse intestines. Journal of Parasitology 67, 450–2.CrossRefGoogle Scholar
Evans, G. A., Margulies, D. H., Camerini-Otero, R. D., Ozato, K. & Seidman, J. G. (1982). Structure and expression of a mouse major histocompatibility antigen gene, H-2L°. Proceedings of the National Academy of Sciences, USA 79, 1994–8.CrossRefGoogle Scholar
Gilliland, G., Perrin, S., Blanchard, K. & Bunn, H. F. (1990). Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proceedings of the National Academy of Sciences, USA 87, 2725–9.CrossRefGoogle ScholarPubMed
Hearne, C. M., McAleer, M. A., Love, J. M., Aitmen, T. J., Cornall, R. J., Ghosh, S., Knight, A. M., Prins, J. B. & Todd, J. A. (1991). Additional microsatellite markers for mouse genome mapping. Mammalian Genome 1, 273–82.CrossRefGoogle ScholarPubMed
Irie, Y. & Iwamura, Y. (1993). Host-related DNA sequences are localized in the body of schistosome adults. Parasitology 107, 519–28.CrossRefGoogle ScholarPubMed
Iwamura, Y. & Irie, Y. (1992). Heterogeneity of host-related DNA sequences in schistosomes. Parasitology Today 8, 90.CrossRefGoogle ScholarPubMed
Iwamura, Y., Irie, Y., Kominami, R., Nara, T. & Yasuraoka, K. (1991). Existence of host-related DNA sequences in schistosome genome. Parasitology 102, 397–403.CrossRefGoogle ScholarPubMed
Jeffereys, A. J., Wilson, V. & Thein, S. L. (1985). Individual -specific ‘fingerprints’ of human DNA. Nature, London 316, 760–9.Google Scholar
Kominami, R., Muramatsu, M. & Moriwaki, K. (1983). A mouse type 2 Alu sequence (M2) is mobile in the genome. Nature, London 301, 87–9.CrossRefGoogle ScholarPubMed
Levy, R. B. & Hansen, T. H. (1980). Functional studies of the products of H-2L locus. Immunogenetics 10, 7–17.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Ozato, K., Evans, G. A., Shykind, B., Margulies, D. H. & Seidman, J. G. (1983). Hybrid H-2 histocompatibility gene products assign domains recognized by alloreactive T cells. Proceedings of the National Academy of Sciences, USA 80, 2040–3.CrossRefGoogle ScholarPubMed
Pullen, J. K., Horton, R. M., Cai, Z. & Pease, L. R. (1992). Structural diversity of the classical H-2 genes: K, D, and L. Journal of Immunology 148, 953–67.CrossRefGoogle ScholarPubMed
Sher, A., Hall, B. F. & Vadas, M. A. (1978). Acquisition of murine major histocompatibility complex gene products by schistosomula of Schistosoma mansoni. Journal of Experimental Medicine 148, 46–57.CrossRefGoogle ScholarPubMed
Simpson, A. J. G., Singer, D., McCutchan, T. F., Sacks, D. L. & Sher, A. (1983). Evidence that schistosome MHC antigens are not synthesized by the parasite but are acquired from the host as intact glycoproteins. Journal of Immunology 131, 962–5.CrossRefGoogle Scholar
Smit, V. T., Boot, A. J., Smits, A. M., Fleuren, G. J., Cornelisse, C. J. & Bos, J. L. (1988). K-ras codon 12 mutations occur very frequently in pancreatic adenocarcinomas. Nucleic Acids Research 16, 7773–82.CrossRefGoogle Scholar
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory host with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle Scholar
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society of London, B 171, 483–93.Google ScholarPubMed
Tanaka, M., Iwamura, Y., Amanuma, H., Irie, Y., Watanabe, M., Watanabe, T., Uchiyama, Y. & Yasuraoka, K. (1989). Integration and expression of murine retrovirus-related sequences in schistosomes. Parasitology 99, 31–8.CrossRefGoogle ScholarPubMed
Vadas, M. A., Butterworth, A. E., Burakoff, S. & Sher, A. (1979). Major histocompatibility complex products restrict the adherence of cytolytic T lymphocytes to minor histocompatibility antigens or to trinitrophenyl determinants on schistosomula of Schistosoma mansoni. Proceedings of the National Academy of Sciences, USA 76, 1982–5.CrossRefGoogle ScholarPubMed