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Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Published online by Cambridge University Press:  06 April 2009

P. A. O. Majiwa ,
R. Thatthi ,
S. K. Moloo ,
J. H. P. Nyeko ,
L. H. Otieno  and
S. Maloo
P. A. O. Majiwa
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
R. Thatthi
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
S. K. Moloo
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
J. H. P. Nyeko
Affiliation:
International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya
L. H. Otieno
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
S. Maloo
Affiliation:
Kenya Agricultural Research Institute (KART)/International Livestock Centre for Africa (ILCA), P.O. Box 80147, Mombasa, Kenya
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Summary

Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

Keywords

digoxigeninpolymerase chain reactiontrypanosomestsetse vector
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 313 - 322
Copyright
Copyright © Cambridge University Press 1994

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References

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