Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T08:46:08.208Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

Published online by Cambridge University Press:  12 March 2019

Xi Zhang ,
Xiu Hong ,
Jiang Yang Duan ,
Lu Lu Han ,
Zi Yang Hong ,
Peng Jiang ,
Zhong Quan Wang  and
Jing Cui Open the ORCID record for Jing Cui [Opens in a new window]
Xi Zhang
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Xiu Hong
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Jiang Yang Duan
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Lu Lu Han
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Zi Yang Hong
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Peng Jiang
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Zhong Quan Wang*
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
Jing Cui*
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China
*
Authors for correspondence: Zhong Quan Wang, E-mail: wangzq@zzu.edu.cn and Jing Cui, E-mail: cuij@zzu.edu.cn
Authors for correspondence: Zhong Quan Wang, E-mail: wangzq@zzu.edu.cn and Jing Cui, E-mail: cuij@zzu.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The plerocercoid (sparganum) of Spirometra erinaceieuropaei is the main aetiological agent of human sparganosis. To improve the current knowledge on S. erinaceieuropaei evolution, we performed multi-locus microsatellite typing of sparganum isolates from China for the first time. All available expressed sequence tag (EST) sequences for the Spirometra were downloaded from the GenBank. The identification and localization of microsatellites in ESTs was accomplished by MISA. Based on the selected microsatellites, the genetic structure of 64 sparganum isolates collected from 11 geographical locations in southwest China were investigated through principal component analysis, STRUCTURE analysis and neighbour-joining clustering. A total of 522 non-redundant ESTs containing 915 simple sequence repeats were identified from 12 481 ESTs screened. Five primer pairs were finally selected. Using these loci, a total of 12 alleles were detected in 64 sparganum isolates. Little variability was observed within each of geographical population, especially among isolates derived from Kunming of Yunnan (YN-KM) province. Both STRUCTURE analysis and the clustering analysis supported that two genotypes existed among the sparganum isolates from southwest China. In conclusion, five microsatellite markers were successfully developed, and sparganum population was observed to harbour low genetic variation, further investigation with deeper sampling was needed to elucidate the population structure.

Keywords

Chinagenetic structuremicrosatellitesparganumSpirometra
Type
Research Article
Information
Parasitology , Volume 146 , Issue 7 , June 2019 , pp. 947 - 955
Check for updates
Copyright
Copyright © Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aggarwal, RK, Hendre, PS, Varshney, RK, Bhat, PR, Krishnakumar, V and Singh, L (2007) Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species. Theoretical and Applied Genetics 114, 359–372.Google Scholar
Almeida, GG, Coscarelli, D, Melo, MN, Melo, AL and Pinto, HA (2016) Molecular identification of Spirometra spp. (Cestoda: Diphyllobothriidae) in some wild animals from Brazil. Parasitology International 65, 428–431.Google Scholar
Anantaphruti, MT, Nawa, Y and Vanvanitchai, Y (2011) Human sparganosis in Thailand: an overview. Acta Tropica 118, 171–176.Google Scholar
Balloux, F and Lugon-Moulin, N (2002) The estimation of population differentiation with microsatellite markers. Molecular Ecology 11, 155–165.Google Scholar
Bazsalovicsova, E, Koleničová, A, Králova-Hromadová, I, Minarik, G, Šoltys, K, Kuchta, R and Štefka, J (2018) Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Lühe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening. Molecular and Biochemical Parasitology 225, 1–3.Google Scholar
Bouck, A and Vision, T (2007) The molecular ecologist's guide to expressed sequence tags. Molecular Ecology 16, 907–924.Google Scholar
Earl, DA and vonHoldt, BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4, 359–361.Google Scholar
Ellis, JR and Burke, JM (2007) EST-SSRs as a resource for population genetic analyses. Heredity 99, 125–132.Google Scholar
Eom, KS, Park, H, Lee, D, Choe, S, Kim, KH and Jeon, HK (2015) Mitochondrial genome sequences of Spirometra erinaceieuropaei and S. decipiens (cestoidea: Diphyllobothriidae). Korean Journal of Parasitology 53, 455–463.Google Scholar
Excoffier, L and Lischer, HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10, 564–567.Google Scholar
Falush, D, Stephens, M and Pritchard, JK (2003) Inference of population structure using multilocus genotype data: Linked loci and correlated allele frequencies. Genetics 164, 1567–1587.Google Scholar
Garbarino, C, Interisano, M, Chiatante, A, Marucci, G, Merli, E, Arrigoni, N, Cammi, G, Ricchi, M, Tonanzi, D, Tamba, M, La Rosa, G and Pozio, E (2017) Trichinella spiralis a new alien parasite in Italy and the increased risk of infection for domestic and wild swine. Veterinary Parasitology 246, 1–4.Google Scholar
Gelanew, T, Hailu, A, Schőnian, G, Lewis, MD, Miles, MA and Yeo, M (2014) Multilocus sequence and microsatellite identification of intra-specific hybrids and ancestor-like donors among natural Ethiopian isolates of Leishmania donovani. International Journal for Parasitology 44, 751–757.Google Scholar
Hubisz, MJ, Falush, D, Stephens, M and Pritchard, JK (2009) Inferring weak population structure with the assistance of sample group information. Molecular Ecology Resources 9, 1322–1332.Google Scholar
Jakobsson, M and Rosenberg, NA (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics (Oxford, England) 23, 1801–1806.Google Scholar
Jeon, HK, Park, H, Lee, D, Choe, S, Kim, KH, Sohn, WM and Eom, KS (2016) Genetic identification of Spirometra decipiens plerocercoids in terrestrial snakes from Korea and China. Korean Journal of Parasitology 54, 181–185.Google Scholar
Kuchta, R and Scholz, T (2017) Diphyllobothriidea. In Caira, JN and Jensen, J (eds), Tapeworms from Vertebrate Bowels of the Earth 2008–2017. Lawrence, Kansas, USA: University of Kansas, Natural History Museum, Special Publication No. 25, pp. 167–189.Google Scholar
Kuchta, R, Scholz, T, Brabec, J and Wicht, B (2015) Chapter 16. Diphyllobothrium, Diplogonoporus and Spirometra. In Xiao, L, Ryan, U and Feng, F (eds), Biology of Foodborne Parasites. Section III Important Foodborne Helminths, FL, USA: CRC Press, pp. 299–326.Google Scholar
La Rosa, G, Marucci, G, Rosenthal, BM and Pozio, E (2012) Development of a single larva microsatellite analysis to investigate the population structure of Trichinella spiralis. Infection, Genetics and Evolution 12, 369–376.Google Scholar
La Rosa, G, Calero-Bernal, R, Pérez-Martín, JE, Tonanzi, D, Galati, F, Serrano-Aguilera, FJ, Rosenthal, BM and Pozio, E (2018) Rare but evolutionarily consequential outcrossing in a highly inbred zoonotic parasite. International Journal for Parasitology 48, 543–553.Google Scholar
Liu, GH, Li, C, Yuan, J, Zhou, DH, Xiong, RC, Lin, RQ, Zou, FC and Zhu, XQ (2012) Characterization of the complete mitochondrial genome sequence of Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae) from China. International Journal of Biological Sciences 8, 640–649.Google Scholar
Liu, Q, Li, MW, Wang, ZD, Zhao, GH and Zhu, XQ (2015) Human sparganosis, a neglected food borne zoonosis. Lancet Infectious Diseases 15, 1226–1235.Google Scholar
Okamoto, M, Iseto, C, Shibahara, T, Sato, MO, Wandra, T, Craig, PS and Ito, A (2007) Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequences. Parasitology International 56, 235–238.Google Scholar
Rousset, F (2008) Genepop'007: a complete re-implementation of the genepop software for Windows and Linux. Molecular Ecology Resources 8, 103–106.Google Scholar
Smouse, PE, Whitehead, MR and Peakall, R (2015) An informational diversity framework, illustrated with sexually deceptive orchids in early stages of speciation. Molecular Ecology Resources 15, 1375–1384.Google Scholar
Steinauer, ML, Blouin, MS and Criscione, CD (2010) Applying evolutionary genetics to schistosome epidemiology. Infection, Genetics and Evolution 10, 433–443.Google Scholar
Swofford, DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. Sinauer Associates, Sunderland, MA.Google Scholar
Tang, TH, Wong, SS, Lai, CK, Poon, RW, Chan, HS, Wu, TC, Cheung, YF, Poon, TL, Tsang, YP, Tang, WL and Wu, AK (2017) Molecular identification of Spirometra erinaceieuropaei tapeworm in cases of human sparganosis, Hong Kong. Emerging Infectious Diseases 23, 665–668.Google Scholar
Varshney, RK, Graner, A and Sorrells, ME (2005) Genic microsatellite markers in plants: features and applications. Trends in Biotechnology 23, 48–55.Google Scholar
Wei, T, Zhang, X, Cui, J, Liu, LN, Jiang, P and Wang, ZQ (2015) Levels of sparganum infections and phylogenetic analysis of the tapeworm Spirometra erinaceieuropaei sparganum in wild frogs from Henan Province in central China. Journal of Helminthology 89, 433–438.Google Scholar
Zhang, X, Wang, H, Cui, J, Jiang, P, Lin, ML, Zhang, YL, Liu, RD and Wang, ZQ (2016) The phylogenetic diversity of Spirometra erinaceieuropaei isolates from southwest China revealed by multi genes. Acta Tropica 156, 108–114.Google Scholar
Zhang, X, Duan, JY, Shi, YL, Jiang, P, Zeng, DJ, Wang, ZQ and Cui, J (2017) Comparative mitochondrial genomics among Spirometra (cestoda: Diphyllobothriidae) and the molecular phylogeny of related tapeworms. Molecular Phylogenetics and Evolution 117, 75–82.Google Scholar
Zhang, X, Shi, YL, Han, LL, Xiong, C, Yi, SQ, Jiang, P, Wang, ZX, Shen, JL, Cui, J and Wang, ZQ (2018) Population structure analysis of the neglected parasite Thelazia callipaeda revealed high genetic diversity in Eastern Asia isolates. PLoS Neglected Tropical Diseases 12, e0006165.Google Scholar
Supplementary material: File

Zhang et al. supplementary material

Tables S1-S2 and Figure S1

Download Zhang et al. supplementary material(File)
File 1.6 MB