Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-26T07:19:52.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing

Published online by Cambridge University Press:  06 April 2009

F. M. Awad-El-Kariem ,
H. A. Robinson ,
D. A. Dyson ,
D. Evans ,
S. Wright ,
M. T. Fox  and
V. Mcdonald
F. M. Awad-El-Kariem
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
H. A. Robinson
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
D. A. Dyson
Affiliation:
Veterinary Investigation Centre, Ministry of Agriculture, Fisheries and Food, Calthwaite, Penrith, Cumbria CA11 9RR
D. Evans
Affiliation:
Clinical Sciences and Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
S. Wright
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH
M. T. Fox
Affiliation:
Department of Pathology, Royal Veterinary College, Royal College Street, London NW1
V. Mcdonald
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
Get access Rights & Permissions [Opens in a new window]

Summary

Isoenzyme typing was used to study a number of oocyst isolates of Cryptosporidium parvum from different geographical locations and of human or animal origin. All isolates showed identical enzyme motility when glucose phosphate isomerase (GPI; 23 isolates tested) or lactate dehydrogenases (LDH; 20 isolates tested) was assayed. However, two isoenzyme forms were observed with phosphoglucomutase (PGM; 9 animal isolates showed one form, while 8/9 human isolates showed a second form) and hexokinase (HK; 4 human isolates showed one form and 6 animal isolates showed a second form). Thus, PGM and HK each exhibit 2 isoenzymes corresponding to 2 parasite populations associated with separate hosts. The data from this study, plus supportive evidence obtained by different methods and by independent researchers, lend support to the hypothesis that separate cycles of transmission of C. parvum may exist within human and animal hosts.

Keywords

Cryptosporidium parvumisoenzyme electrophoresiszoonotic transmissionhost specificity
Type
Research Article
Information
Parasitology , Volume 110 , Issue 2 , February 1995 , pp. 129 - 132
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alpert, G., Bell, L. M., Kirkpatrick, C. E., Budnick, L. D., Campos, J. M., Friedman, L. M. & Plotkin, S. A. (1986). Outbreak of cryptosporidiosis in a day-care center. Pediatrics 77, 152–7.CrossRefGoogle Scholar
Awad-El-Kariem, F. M., Robinson, H. A., Mcdonald, V., Evans, D. & Dyson, D. A. (1993). Is human cryptosporidiosis a zoonotic disease? Lancet 341, 1535.CrossRefGoogle Scholar
Casemore, D. P. (1989). Cryptosporidiosis: another source. British Medical Journal 298, 750–1.CrossRefGoogle ScholarPubMed
Casemore, D. P. & Jackson, F. B. (1984). Hypothesis: cryptosporidiosis in human beings is not primarily a zoonosis. Journal of Infection 9, 153–6.CrossRefGoogle Scholar
Evans, D. A., Lanham, S. M., Baldwin, C. I. & Peters, W. (1984). The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 35–42.CrossRefGoogle ScholarPubMed
Godfrey, D. G. & Kilgour, V. K. (1976). Enzyme electrophoresis in characterising the causative organism of Gambian trypanosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 219–24.CrossRefGoogle ScholarPubMed
Højlyng, N., Mølbak, K. & Jepsen, S. (1985). Cryptosporidiosis in human beings is not primarily a zoonosis. Journal of Infection 11, 270–1.CrossRefGoogle Scholar
Mccarthy, M. (1993). USA: cryptosporidiosis outbreak. Lancet 341, 1084.CrossRefGoogle Scholar
Mcdonald, V., Stables, R., Warhurst, D. C., Barer, M. R., Blewett, D. A., Chapman, H. D., Connolly, G. M., Chiodini, P. L. & Mcadam, K. P. W. J. (1990). In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs. Antimicrobial Agents and Chemotherapy 34, 1498–500.CrossRefGoogle ScholarPubMed
Miron, D., Kenes, J. & Dagan, R. (1991). Calves as a source of an outbreak of cryptosporidiosis among young children in an agricultural closed community. Pediatric Infectious Disease Journal 10, 438–41.CrossRefGoogle Scholar
Nina, J. M. S., McDonald, V., Deer, R. M. A., Wright, S. E., Dyson, D. A., Chiodini, P. L. C. & Mcadam, K. P. W. J. (1992). Comparative study of antigenic composition of oocyst isolates of Cryptosporidium parvum from different hosts. Parasite Immunology 14, 227–32.CrossRefGoogle ScholarPubMed
Ogunkolade, B. W., Robinson, H. A., McDonald, V., Webster, K. & Evans, D. A. (1993). Isoenzyme variation within the genus Cryptosporidium. Parasitology Research 79, 385–8.CrossRefGoogle ScholarPubMed
Ortega, Y. R., Sheehy, R. R., Cama, V. A., Oishi, K. K. & Sterling, C. R. (1991). Restriction fragment length polymorphism analysis of Cryptosporidium parvum isolates of bovine and human origin. Journal of Protozoology 38, 540–1.Google ScholarPubMed
Phls Study Group (1990). Cryptosporidiosis in England and Wales: prevalence and clinical and epidemiological features. British Medical Journal 300, 774–7.CrossRefGoogle Scholar
Pozio, E., Gomez, Morales M. A., Barbieri, F. M. & La Rosa, G. (1992). Cryptosporidium: different behaviour in calves of isolates of human origin. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 636–8.CrossRefGoogle ScholarPubMed
Shirley, M. W. (1986). New methods for the identification of species and strains of Eimeria. In Research in Avian Coccidiosis (ed. Mcdougald, L. R., Joyner, L. P. & Long, P. L.). Georgia: University of Georgia Press.Google Scholar
Smith, H. V. & Rose, J. B. (1990). Waterborne cryptosporidiosis. Parasitology Today 6, 8–12.CrossRefGoogle ScholarPubMed
Walliker, D. (1985). Characterization of Plasmodium falciparum of different countries. Annales de la Société Belge de Médecine Tropicale 65, 69–77.Google ScholarPubMed